PURPOSE To compare different preparation methods for a suitable amniotic membrane (AM) extract containing a given amount of growth factors. METHODS In this interventional case series, we dissected the AM from eight placentas within 24 hours after delivery, under clean conditions. After washing and mixing, AM extracts (AMEs) were prepared using pulverization and homogenization methods, and dif...

Background: Ocular chemical burns induce devastating and permanent damage to the ocular surface. Rapid intervention is required for maximal visual rehabilitation. Amniotic membrane transplantation (AMT) may save the ocular surface, however it introduces a potentially unnecessary surgical trauma in such compromised eyes. Amniotic membrane extracts (AME) could be a practical substitute of AMT in ...

PURPOSE Surgery to reconstruct the ocular surface is greatly facilitated by the use of amniotic membrane, either as a biologic drape or, more recently, as a substrate for the transplantation of cultivated corneal epithelial cells. This study was designed to compare the usefulness of intact and denuded human amniotic membranes as a substrate for corneal epithelial cell culture. METHODS Small (...

purpose: to compare outcomes and recurrence rates of amniotic membrane transplantation (amt) plus intraoperative mitomycin c (mmc) and conjunctival rotational autograft (cra) plus intraoperative mmc in pterygium surgery methods: ninety-two eyes of 92 patients with primary nasal pterygium were enrolled. all patients were randomized to undergo either amt or cra. forty-three patients underwent amt...

PURPOSE The immunogenic characterization of amniotic membrane is still unknown. This study was designed to examine the immunogenicity of human amniotic membrane, by using experimental xenotransplantation models. METHODS Anti-human class I, class II, and Fas ligand monoclonal antibodies were used against cryopreserved amniotic membrane and cell viability tested for cryopreserved amniotic membr...

background & objectives: one of the important infectious factors in pregnant mothers and newborns is group b streptococcus (gbs). there is no perfect report about prevalence of gbs in iran and in the case of preterm rupture of amniotic membrane or preterm labor all patients are treated by antibiotics without culture so this has led to adverse taking antibiotics and drug resistance. the pres...

Amniotic membrane is applied to the diseased ocular surface to stimulate wound healing and tissue repair, because it releases supportive growth factors and cytokines. These effects fade within about a week after application, necessitating repeated application. Generally, amniotic membrane is fixed with sutures to the ocular surface, but surgical intervention at the inflamed or diseased site can...

purpose: to develop a nanofibrous polycaprolacton (pcl) substrate for limbal stem cell (lsc) expansion that can serve as a potential alternative substrate to replace human amniotic membrane. methods: the human limbal stem cells (lscs) were used to evaluate the biocompatibility of substrates (nanofibrous scaffold, and human amniotic membrane) regarding cellular phenotypic profile, viability, pro...

Cryopreserved amniotic membrane modulates adult wound healing by promoting epithelialization while suppressing stromal inflammation, angiogenesis and scarring. Such clinical efficacies of amniotic membrane transplantation have been reported in several hundred publications for a wide spectrum of ophthalmic indications. The success of the aforementioned therapeutic actions prompts investigators t...