Protein phosphorylation and dephosphorylation systems modulate many cellular activities and have recently been implicated in the in vitro transport of newly synthesized proteins. Here we show that polarized transport from the Golgi to the plasma membrane in intact MDCK cells is regulated by phosphorylation-dephosphorylation. Transport is inhibited by the phosphatase inhibitor okadaic acid and i...

INTRODUCTION Trace elements are known to influence bone metabolism; however, their effects may be exacerbated in renal failure because dialysis patients are unable to excrete excess elements properly. Our study correlated bone quality in dialysis patients with levels of bone fluoride, magnesium, and aluminum. A number of studies have linked trace elements, including fluoride, magnesium, and alu...

The advancement of far-ultraviolet (FUV) coatings is essential to meet the specified throughput requirements of the Large UV/Optical/IR (LUVOIR) Surveyor Observatory which will cover wavelengths down to the 100 nm range. The biggest constraint in the optical thin film coating design is attenuation in the Lyman-Alpha Ultraviolet range of 100-130 nm in which conventionally deposited thin film mat...

Aluminum fluoride may be used both to stabilize microtubules and to induce strong binding of kinesin, thus circumventing the need for taxol and AMP-PNP in kinesin preparations.

Fluoride removal from aqueous solutions using a-Al2O3 as an adsorbent was investigated through adsorption and electrokinetic studies, using two different initial fluoride concentrations relevant to fluoride contents in drinking water, namely 3 and 10 mg/L. Maximum fluoride removal was achieved between pH 5 and 6 at 258C. Adsorption at both pH 5 and 9 correlates to the Langmuir isotherm. The poi...

Targeted agents are increasingly used for treating cancer and other diseases, but patients may need to be carefully selected to maximize the potential for therapeutic benefit. One way to select patients is to bind an imaging radionuclide to a targeting agent of interest, so that its uptake in specific sites of disease can be visualized by positron-emission tomography (PET) or single-photon emis...

The concentration of [3H]DHA was 1.0 n M . Non-specific binding was defined by the curve-fitting program and it was determined as 29% of total [”IDHA binding. The competition curve fit best to a two-site model (f.’<O.0025), with K , = 4 f 2 nM and K L = 3 f 2 ,UM ( m e a n s + s ~ ~ of three separate competition experiments which were pooled). These values were obtained by setting the affinity ...

Fluoride has long been known to influence the activity of various enzymes in vitro. Latterly it has been demonstrated that many effects primarily attributed to fluoride are caused by a synergistic action of fluoride plus aluminum. Fluorinated chemicals are of growing importance, with applications in medicine. Fluorine substitution has profound effects on the properties of organic compounds. The...

Responses of adipose cells to adrenoceptor regulation, including that of β-adrenoceptor (AR), and the signalling machinery involved in these responses are not sufficiently understood; information that is helpful for elucidating the adrenoceptor (adrenergic and β-AR)-responsive machinery is insufficient. We examined phospho-Thr-172 AMPK production in mouse-derived 3T3-L1 adipocytes treated with ...