Objective: Investigations were carried out to isolate bacteria from saline-alkali soils and determined optimized alkaline protease activity and stability produced by a wild strain of bacillus sp. in submerged fermentation (SMF). Methods: Optimum temperature for enzyme activity in the crude extract was 40 ◦C at a pH between 8.0 and 9.0. The studies on pH stability showed that the enzyme...

The yeast-like fungus Aureobasidium pullulans PL5 is a microbial antagonist against postharvest pathogens of fruits. The strain is able to produce hydrolases, including glucanases, chitinases and proteases. The alkaline serine protease gene ALP5 from A. pullulans was cloned, inserted into the vector pPIC9 to construct pPIC9/ALP5, and then expressed in Pichia pastoris strain KM71. ALP5 had a mol...

This paper reported the purification and kinetic parameters characterization of an alkaline protease produced from Bacillus subtilis through submerged fermentation process using rice husk as growth supporting substrate (by-product of rice industry) collected from Pearl Rice Mill Hafiz Abad, Faisalabad. Bacillus subtilis was cultured in fermentation medium under some pre-optimized growth conditi...

Fungal strains isolated from poultry farm soil by Waksman serial dilution method were screened for alkaline protease production. Isolate PF/F/107, the most potent producer of alkaline protease was identified as Aspergillus versicolor. The isolate PF/F/107 showed highest activity in the optimized medium I at pH 9.0, temperature 35oC, with 1% wheat bran and 1% sodium nitrate incubated for 4 days.

An alkaline protease-producing fungus was screened from potato grown soil fields of Bangalore and identified as Aspergillus terreus gr. based on morphological and microscopic features. The selected growth medium contained casein (1%) as a sole source of carbon and nitrogen. The pH of the medium was adjusted to 10.0 using sodium carbonate in order to isolate alkalophilic fungi. The best process ...

background and objectives: proteases are a group of enzymes that catalyze the degradation of proteins resulting in the pro- duction of their amino acid constituents. they are the most important group of industrial enzymes which account for about 60% of total enzymes in the market and produced mainly by microorganisms. the attempts were made to study the kinetic parameters of protease produced b...

A nonlysosomal alkaline protease which degrades the oxidatively modified form of Escherichia coli glutamine synthetase has been purified to apparent homogeneity from rat and mouse liver acetone powders. Its molecular weight was determined to be 300,000 by Sephacryl S-300 gel filtration but results of further studies using high pressure liquid chromatography gel filtration suggest a value of 650...