After albumin transfusion levels of alkaline phosphatase are raised. These levels were studied in selected groups of patients and in albumin prepared for transfusion using a heat sensitivity test, and by this means the significance of raised levels of alkaline phosphatase may be interpreted correctly.

We have proposed the use of the fluorimetric method with 4-methylumbelliferyl phosphate (4-MUP) specific substrate for the alkaline phosphatase determination in the neutrophil adhesion assay. We provide evidence that the endogenous neutrophil alkaline phosphatase (NAP) activity evaluation is reliable to quantify neutrophil adhesion at a wide range of cell numbers (10(4)-10(6)). The results obta...

The FLAG peptide, AspTyrLysAspAspAspAspLys, has been used as an epitope tag in a variety of cell types. The modification of the cytomegalovirus (CMV) promoter containing vector, pCMV5, to create two transient expression vectors designed for secretion and intracellular expression of FLAG-fusion proteins in mammalian cells is described. As a functional test, the bacterial alkaline phosphatase gen...

We compared alkaline phosphatase (EC 3.1.3.1) and gamma-glutamyltransferase (EC 2.3.2.2) activities in sera from children with various hepatic diseases with values for normal children. Although values for both enzymes are above normal in essentially the same liver disorders, the latter is much more specific and sensitive. A single value for alkaline phosphatase, measured at any time during chil...

This investigation concerns identification of alkaline phosphatase production by bacterial organisms, as detected by a blue color resulting from conversion of indolyl phosphate to indigo. Coagulase-positive Staphylococcus produced alkaline phosphatase; coagulase-negative Staphylococcus did not. Serratia did not produce alkaline phosphatase; those Enterobacteriaceae we tested did. Thus, this tes...

Enzymes associated with release of iron from internalized ferrated siderophore (ferrisiderophore reductase), with damage to the cell at high iron concentration (superoxide dismutase) and siderophore synthesis (alkaline phosphatase), were examined in 3 test fungi viz., Aspergillus sp. ABp4, Aureobasidium pullulans and Rhizopus sp. Extracellular ferrisiderophore reductase activity was present in ...

conclusions our findings clearly indicate a synergistic effect of waterpipe smoke, immobilization and darkness stress on serum ck levels, whereas serum alp levels of experimental groups have shown no significant synergism for the mentioned factors. results the results indicated enhanced serum ck and alp levels in all groups compared with control (p < 0.05). also, there was a significant differe...

despite the availability of various alkaline phosphatase (alp) isoenzymes, the calf enzyme is being used in current enzyme assays as the detector enzyme. the glycosylation pattern of this enzyme makes it a suitable ligand for binding to wheat germ agglutinin lectin (wga). as a result of this property, the enzyme can not be used as a conjugate with this lectin, and the calf enzyme conjugates can...