background: to investigate the effect of cana2-edta and experimental diabetes (iddm) on zinc and carbohydrate metabolism and the activities of got, gpt and alkaline phosphatase. methods: forty male normal albino (wistar) rats of 8 weeks of age were fed with a basal diet. twenty rats were then intraperitoneally injected with alloxan to induce diabetes. then after one week ten rats from each grou...

conclusions the elevation of hmw-alp activity in cu treated animal suggests the occurrence of biliary disease. this may be used as a biomarker for the diagnosis of copper toxicity. results obtained data showed that with increasing administration of copper, the alp activity was elevated significantly. in comparison with the control group the elevations were between 20%-56% using gel filtration c...

introduction: salivary calcium and phosphate levels might have a relationship with dental caries severity. evaluation of different indexes such as alkaline phosphatase, which increases salivary phosphate levels, and hydroxyapatite ion activity, which reflects salivary calcium and phosphate levels, might help determine the risk of dental caries. the aim of the present study was to compare saliva...

A monoclonal antibody to digoxin enabled sandwich techniques to be used for the detection of hybridised digoxigenin labelled probes in cultured cells and paraffin wax sections. This system has greater flexibility than alkaline phosphatase conjugated polyclonal antidigoxigenin antibody and permits the use of alternative detector enzymes, such as horseradish peroxidase and fluorescence labels. Th...

AIMS To examine thymomas for proteins encoded by oncogenes and to determine whether their presence correlates with tumour growth and associated myasthenia gravis. METHODS Sections of 24 thymomas were incubated with anti-EGF receptor (EGF-R), anti-Ki67 antigen, anti-p53, and anti-bcl-2 antibodies, and then stained using the alkaline phosphatase/anti-alkaline phosphatase (APAAP) technique. Cell...

objective: different techniques have been suggested for the repair of bone defects at the injured sites. use of biomembranes, or application of plasma rich in growth factor (prgf) at the site of proliferation of osteoblasts are among the suggested techniques. the current study aimed to compare the biocompatibility of human periodontal ligament fibroblasts (hplf) cultured on hypro-sorb f, perica...

Thirty four patients positive for human immunodeficiency virus (HIV) who had lymphadenopathy were investigated using fine needle aspiration. Cytological analysis included immunocytochemical investigation with the alkaline phosphatase-antialkaline phosphatase (APAAP) method. All patients had confirmation of cytological diagnosis by lymph node biopsy. Fifteen aspirates with follicular hyperplasia...

alkaline phosphatase (ortho phosphate, monoester hydrolase phosphoinositide e. c. 3.1.3.1) is a non-specific metalloproteinase enzyme that is located inside periplasmic space of bacteria. this enzyme is used to measure freshwater sediment in genetic engineering for cleaning water. isolation and molecular identification of the bacteria producing alkaline phosphatase and comparison of its product...

introduction: hydatid cyst disease is caused by the protoscolices of echinococcus granulosus. alkaline phosphatase (alp) enzyme is required for metabolism, physiology, immunology, and nutrients absorption in parasite. the aim of this study was to compare the level of alp activity (as a pathological biomarker) in hydatid cyst protoscolices (hcp) with that of sheep liver tissue and to determine t...