When isolated hepatocytes are incubated with phosphatidylinositol-specific phospholipase C, three cell-surface enzymes show markedly different behaviour. Most of the alkaline phosphatase is released at very low values of phosphatidylinositol hydrolysis, whereas further phosphatidylinositol hydrolysis releases only a maximum of about one-third of the 5'-nucleotidase. Alkaline phosphodiesterase I...

Escherichia coli alkaline phosphatase (AP) is the prototypical two metal ion catalyst with two divalent zinc ions bound approximately 4 A apart in the active site. Studies spanning half a century have elucidated many structural and mechanistic features of this enzyme, rendering it an attractive model for investigating the potent catalytic power of bimetallic centers. Unfortunately, fundamental ...

M i l d alkaline hydrolysis of phospholipids, a method introduced by Dawson ( l ) , is based on the following principle. The carboxyl ester groups of a phospholipid are much more susceptible to nucleophilic attack by hydroxy or alcoholate ions than the phosphate group. Thus, by choosing appropriate concentrations of base, phospholipids can be deacylated (Le., the fatty acid esters can be prefer...