We report another patient with a circulating alkaline phosphatase/immunoglobulin complex in his blood, and describe a simple method of demonstrating such complexes. On electrophoresis on cellulose acetate, the complex was relatively slow moving and there was no activity in the normal bone/liver isoenzyme region. When the serum was treated with trypsin, the slow band disappeared and the normal p...

alkaline phosphatase closely resembled that extracted from microsomes of human liver in some physicochemical respects. The specific activities of these enzymes in the complex were found to be of similar order of magnitude as compared with those of the same enzymes in plasma membranes isolated from the human liver. From the precipitate formed by incubating the complex with its antibody, all the ...

Light-absorption, c.d. and fluorescence of the bilirubin-albumin complex were investigated at extreme alkaline pH. Above pH 11.1 albumin binds the bilirubin molecule, twisted oppositely to the configuration at more neutral pH. On the basis of light-absorption it is shown that two alkaline transitions occur. The first alkaline transition takes place at pH between 11.3 and 11.8, co-operatively di...

The increased alkaline phosphatase (EC 3.1.3.1) activity of reconstituted lyophilized control sera has recently been the focus of considerable interest because of its possible implications for quality-control procedures. We confirm that these reconstituted materials show a temperature-dependent increase in alkaline phosphatase activity, but could show no alteration in activity of fresh sera. Th...

Herpes simplex virus encodes seven proteins which have been shown to be both necessary and sufficient for in vitro replication of origin-containing plasmids. We have shown previously that one of these proteins, the major DNA-binding protein mDBP, forms a complex with alkaline nuclease, which is not one of the seven essential proteins. In this study, we have employed immunological reagents and a...

Pichagchi pluton is situated in the northeastern part of the Soursat complex in Sanandaj-Sirjan metamorphic belt of Iran. The pluton with the age of 74.20 Ma is composed mainly granodiorite, diorite, and tonalite in which mafic minerals are biotite and amphibole. In this study, composition of minerals used to describe the nature of the granitic magma and estimate the pressure and temperature at...

HYDROGEN PEROXIDE HAS ALWAYS been a technologically attractive oxidant to the pulp and paper industry, with its significance becoming increasingly important as environmental regulations become more stringent. Beyond its ease of handling and application, hydrogen peroxide is also a versatile and benign compound whose decomposition products are water and oxygen. Among its major drawbacks are its ...

The complex formation between mercury(II) and penicillamine (H(2)Pen = 3,3'-dimethyl cysteine) in alkaline aqueous solutions (pH approximately 2) has been investigated with extended X-ray absorption fine structure (EXAFS) and 199Hg NMR spectroscopy. By varying the penicillamine concentration (C(H(2)Pen) = 0.2-1.25 M) in approximately 0.1 M Hg(II) solutions, two coexisting major species [Hg(Pen)...

Gastric alkaline secretion was determined in ranitidine treated healthy subjects and duodenal ulcer (DU) patients using gastric perfusion aspiration system and back titration of gastric perfusate to original pH 6.0. Basal alkaline secretion showed periodic fluctuations reaching peaks at phase III of the migrating motor complex (MMC) in the stomach. Mean basal alkaline secretion in healthy norma...