نتایج جستجو برای: algd
تعداد نتایج: 85 فیلتر نتایج به سال:
Transcription of algD, encoding GDP-mannose dehydrogenase, the key enzyme in the alginate biosynthetic pathway, is highly regulated in Azotobacter vinelandii. We describe here the characterization of a Tn5 insertion mutant (AC28) which shows a higher level of expression of an algD::lacZ fusion. AC28 cells were morphologically abnormal and unable to encyst. The cloning and nucleotide sequencing ...
In this month’s issue of the Journal of Bacteriology, Jones et al. (1) examine the relationship between the Pseudomonas aeruginosa chronically produced exopolysaccharide, alginate, and a polysaccharide that is produced during the initial stages of biofilm formation, Psl. Psl, composed of mannose, rhamnose, and glucose, was identified as one of the first exopolysaccharides in nonmucoid biofilm m...
بیش از %90 موارد عفونت های مجاری ادراری در زنان ناشی از دو میکروارگانیسم ¬اشرشیا کولی¬(escherichia coli) و استافیلوکوک ¬ساپروفیتیکوس (staphylococcus saprophyticus) هستند و حداقل 80 درصد از موارد uti از اشرشیا کولی(e. coli) ناشی می¬شوند. از جمله پاتوژن¬های کمتر شایع می¬توان به کلبسیلا پنومونیه (klebsiella pneumonia)، سودوموناس¬ آئروجینوزا pseudomonas aeruginosa))، انتروکوکوس ¬فکالیس (enterococcu...
Background: The aim of this study was compared the efficacy of the designed primers and already published primers for detection of the exoA, oprL and algD genes by PCR assay for finding a rapid, accurate and highly sensitive and specific procedure to detect the Pseudomonas aeruginosa in the serious and fatal infections such as cystic fibrosis disease, burned individual.Methods: A total of 150...
Groundwater fluoride contamination is one of the dominant toxicological environmental problems in India and overseas. Water samples were taken from Natham taluk screened resistant bacteria. Initially, Twenty- four colonies selected 50 mM NaF supplemented LB agar plates. All isolates showed ?-haemolysis on blood plates, confirmed as pathogens. Virulence factors (algD, plcH, toxA, gyrB, rhlC, las...
زمینه و هدف: سودوموناس آئروژینوزا، یکی از رایجترین عوامل عفونتهای بیمارستانی محسوب میشود. بیشتر آزمایشگاهها بهطور معمول برای شناسایی سودوموناس آئروژینوزا، از روش کشت و تستهای بیوشیمیایی مختلف استفاده میکنند که روشی وقتگیر بوده و در برخی موارد دارای نتایج مثبت و منفی کاذب میباشد. هدف از انجام این پژوهش مقایسه دو روش مولکولی PCR با استفاده از DNA استخراجشده با کیت و PCR با استفاده از کل...
Aim: In this it is aimed to the determine presence of virulence resistance genes (toxA, algD, plcN, lasB, plcH) in P. aeruginosa isolates isolated from blood samples.
 Materials and methods: DNA extraction study was done by boiling method. Optimization using positive control after extraction. After optimization, investigated polymerase chain reaction (PCR) method.
 Results: As a resul...
Background and Objective: Pseudomonas aeroginosa is the most common pathogen associated with nosocomial infections and possesses virulence factors which contribute to the bacterial invasion and toxicity such as alginate, exoenzyme S, exotoxin A and elastase. The aim of this study was to determine the prevalence of algD, exoS, toxA and lasB genes in Pseudomonas aeruginosa samples isolated from p...
Background and aim: Excessive use of antibiotics has significantly increased resistance to common antibiotics in the treatment of urinary tract infections. In the present study, antimicrobial efficacy of chitosan nanoparticles and peptide IDR-1018 was evaluated against P. aeruginosa isolates. Methods: In the present study, 20 resistant and 20 sensitive P. aeruginosa isolates to imipenem were ...
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