By yeast two-hybrid screening using the calcium-binding protein ALG-2 as bait a new target of ALG-2 was identified, the RNA-binding protein RBM22. In order to confirm these interactions in vivo we prepared fluorescent constructs by using the monomeric red fluorescent protein to label ALG-2 and the enhanced green fluorescent protein to label RBM22. Confocal microscopy of NIH 3T3 cells transfecte...

A series of 3 experiments were conducted to determine the in vitro and in situ disappearance, preference, and performance of steers consuming increasing concentrations of a novel feedstuff called algae meal (ALG). In Exp. 1 and 2, 3 ruminally cannulated steers (998 ± 103 kg BW) and 4 treatments were utilized: a dry-rolled corn-based diet (CON), or 15% ALG (A15), 30% ALG (A30), or 45% ALG (A45) ...

Over the last few years, significant research has been conducted in the construction of artificial bone scaffolds. In the present study, different types of polymer scaffolds, such as chitosan-alginate (Chi-Alg) and chitosan-alginate with fucoidan (Chi-Alg-fucoidan), were developed by a freeze-drying method, and each was characterized as a bone graft substitute. The porosity, water uptake and re...

Peflin, a newly identified 30-kDa Ca(2+)-binding protein, belongs to the penta-EF-hand (PEF) protein family, which includes the calpain small subunit, sorcin, grancalcin, and ALG-2 (apoptosis-linked gene 2). We prepared a monoclonal antibody against human peflin. The antibody immunoprecipitated a 22-kDa protein as well as the 30-kDa protein from the lysate of Jurkat cells. Western blotting of t...

Calcium-induced alginate gel beads (Alg-Ca) containing various polysaccharides, including an alginate hydrolysate, were prepared and the drug release profiles were investigated. Hydrocortisone (HC) was gradually released from Alg-Ca into the mimic gastric fluid, while in intestinal fluid, it was quickly released with the dissolution of Alg-Ca. However, with Alg-Ca containing 5% chitin (CT), dis...

Connections between Algebraic Logic and (ordinary) Logic. Algebraic counterpart of model theoretic semantics, algebraic counterpart of proof theory, and their connections. The class Alg(L) of algebras associated to any logic L. Equivalence theorems stating that L has a certain logical property iff Alg(L) has a certain algebraic property. (E.g. L admits a strongly complete Hilbertstyle inference...

Peroxisome proliferator-activated receptor-γ (PPARγ) agonists like pioglitazone (PGZ) are effective antidiabetic drugs, but they induce fluid retention and body weight (BW) gain. Dipeptidyl peptidase IV (DPP IV) inhibitors are antidiabetic drugs that enhance renal Na(+) and fluid excretion. Therefore, we examined whether the DPP IV inhibitor alogliptin (ALG) ameliorates PGZ-induced BW gain. Mal...

A series of 3 experiments were conducted to determine the in vitro and in situ disappearance, preference, and performance of steers consuming increasing concentrations of a novel feedstuff called algae meal (ALG). In Exp. 1 and 2, 3 ruminally cannulated steers (998 ± 103 kg BW) and 4 treatments were utilized: a dry-rolled corn-based diet (CON), or 15% ALG (A15), 30% ALG (A30), or 45% ALG (A45) ...

Immobilization of Raji cells on surface coated with anti-lymphocyte globulin (ALG) at low cell densities lead to the synthesis of Epstein-Barr virus (EBV) early antigen (EA) in up to 5% of the cells. At higher cell densities the percentage of antigen-positive cells decreased and at confluency no antigen synthesis was observed. Addition of iododeoxyuridine (IdUrd) to low density cultures increas...

leishmania antigens are weak immunogens and need to be potentiated by various adjuvants and delivery systems. alginate microspheres as an antigen delivery system and quillaja saponins (qs) as an immunoadjuvant have been used to enhance the immune response against autoclaved leishmania major (alm). microspheres were prepared by an emulsification technique and characterized for size, encapsulatio...