The bacterial leaf spot described here was first recognized on Turkestan and Grimm alfalfa (Medicago sativa L.) in two locations at Madison, Wis., in August 1930. The alfalfa in both instances was in cultivated experimental rows and had been repeatedly splashed bywatering with a garden hose. In October 1931 the disease recurred under similar circumstances at one of these locations, but with the...

Leaf tissue of alfalfa (Medicago sativa L.) was found to contain prolinebetaine, pipecolatebetaine, hydroxyprolinebetaine, and glycinebetaine. As n-butyl esters, these chemical species exhibit molecular cations at mass/charge ratio (m/z) 200, 214, 216, and 174, respectively, when analyzed by fast atom bombardment mass spectrometry. The underivatized betaines exhibit protonated molecular ions at...

In order to obtain a salt-tolerant perennial alfalfa (Medicago sativa L.), we transferred the halophyte Salicornia europaea L. Na(+)/H(+) antiporter gene, SeNHX1, to alfalfa by using the Agrobacterium-mediated transformation method. The transformants were confirmed by both PCR and RT-PCR analyses. Of 197 plants that were obtained after transformation, 36 were positive by PCR analysis using 2 pr...

An adenylate cyclase activity in Medicago sativa L. (alfalfa) roots was partially characterized. The enzyme activity remains in the supernatant fluid after centrifugation at 105,000 g and shows in crude extracts an apparent Mr of about 84,000. The enzyme is active with Mg2+ and Ca2+ as bivalent cations, and is inhibited by EGTA and by chlorpromazine. Calmodulin from bovine brain or spinach leav...

The composition of phosphorus-containing compounds of intact lyophilized alfalfa tissue has been determined, in part, by solid-state (31)P nuclear magnetic resonance. The tissue (Medicago sativa L., cv Regen S; and some of its crosses) was grown in culture under both nonregenerating and regenerating conditions, the latter enhanced by the addition of specific amino acids. Analysis of the (31)P n...

This study was conducted to determine the chemical composition, digestibility, degradability and nutritive value of madder, Rubia tinctorum L.  and alfalfa Medicago sativa. using in vitro and in situ measurements. The mean values for OM, CP, NDF, ADF and EE were 892.5, 172.0, 306.5, 219.4 and 16.0 g/kg DM for madder hay, and 921.3, 141.4, 482.0, 361.1 and 6.50 g/kg DM for alfalfa hay, respectiv...

salinity is among important soil stresses adversely affecting the process of nitrogen (n) fixation in leguminous plants in different parts of the world. it has been indicated that salinity can inhibit the early stages of nodulation process between bacterium and the host plant including the exchange of signal molecules (nod gene inducers). there has not been any research regarding the effects of...

Alfalfa (Medicago sativa L.) biomass was evaluated for biochemical conversion into ethanol using dilute-acid and ammonia pretreatments. The two alfalfa lines compared were a reduced S-lignin transgenic cultivar generated through down regulation of the caffeic acid O-methyltransferase gene and a wild-type control. Both were harvested at two maturities. All the samples had similar carbohydrate co...

this study was conducted to determine the chemical composition, digestibility, degradability and nutritive value of madder, rubia tinctorum l.  and alfalfa medicago sativa. using in vitro and in situ measurements. the mean values for om, cp, ndf, adf and ee were 892.5, 172.0, 306.5, 219.4 and 16.0 g/kg dm for madder hay, and 921.3, 141.4, 482.0, 361.1 and 6.50 g/kg dm for alfalfa hay, respectiv...

A potential strategy to decrease the levels of self-seed production during the seed increase stages of alfalfa (Medicago sativa L.) synthetic cultivar development is selection for decreased self-fertility. The underlying genetics of this trait have not been elucidated and, therefore, a study was designed to identify genetic determinants of alfalfa self-fertility. An F1 mapping population develo...