Among the techniques which have been reported to stain the surface coat of cells, for electron microscopy, is lanthanum staining en bloc. Similarly, the presence of the cationic dye, Alcian blue 8GX, in a primary glutaraldehyde fixative has been reported to improve the preservation of the surface coat of cells of many types; however, the preserved coat is not very electron opaque unless thin se...

Histochemical studies were performed on glycosaminoglycan (GAG) components in Wilms tumors of rats and rabbits and fetal kidneys of rats. 1. Wilms tumors. The interstitial components had an intense stainability with Alcian blue, and digestion test with hyaluronidase and chondroitinase was intensely positive. The epithelial tumor cells, showing a tubular pattern, were also stained with Alcian bl...

Mast cells are particularly in association with structures, especially the skin, respiratory, and digestive systems proximity to surfaces that interface external environment. This study was carry out demonstrate distribution heterogeneity of mast Angora goat glandula mandibularis by using morphological, histochemical, immunohistochemical methods. A total seven healthy male adult goats' mandibul...

introduction: the aim of this study was to demonstrate changes in nature of mucin and production of acidic forms using pas-alcian blue stain at ph=2.5. appearance of non-goblet metaplastic columnar cells of lower esophagus is considered as an early sign of barrette sophagus, simultaneous in conjunction or before the appearance of goblet cells in patients with gastroesophageal reflux disease. ma...

One of the complications Diabetes Mellitus (DM) is damage to kidneys. Using L. plantarum, it can reduce glucose levels so that also possible repair damaged kidney structures. The process repairing structure by L plantarum be seen microscopically in glomerulus with Alcian Blue staining (AB). purpose study was determine differences microscopic results DM rat kidneys after treatment AB staining. s...

The histologic evaluation of endochondral ossification is critical to the study of fracture healing, developmental biology, and comparative histology. A modification of Sayers’ alcian blue hematoxylin staining technique is described in which a different counterstain is applied. Paraffin sections are stained in alcian blue hematoxylin for 30 min, and then placed in orange G-eosin counterstain fo...

A B S T R ACT The relationship between Ca ++ and pinocytosis was investigated in Amoeba proteus. Pinocytosis was induced with 0.01% alcian blue, a large molecular weight dye which binds irreversibly to the cell surface. The timecourse and intensity of pinocytosis was monitored by following the uptake of [all]sucrose. When the cells are exposed to 0.01% alcian blue, there is an immediate uptake ...

Recently, we reported that Campylobacter jejuni, an important gastrointestinal pathogen, has the genetic determinants to produce a capsular polysaccharide (Karlyshev et al., Mol. Microbiol. 35:529-541, 2000). Despite these data, the presence of a capsule in these bacteria has remained controversial. In this study we stain C. jejuni cells with the cationic dye Alcian blue and demonstrate for the...