The expression of albumin and alpha 1-inhibitor 3 genes was investigated in rat cell suspensions enriched in periportal (n = 10) and perivenous (n = 10) hepatocytes obtained by the digitonin-collagenase technique. The degree of enrichment of the cell suspensions was assessed: (1) by enzymic assays for the periportal marker alanine aminotransferase and for the perivenous marker glutamine synthet...

Although the mechanisms under which sex ratio is controlled in nature remain unknown, researchers have attempted to treat sperm in an effort to predetermine the sex of the offspring. The methods of sperm separation that have been repeatedly reported to isolate fractions rich in X- or Y-chromosome bearing sperm are serum albumin gradient swim-up and flow cytometry. However, the ability of the se...

The concentration of protein in the cerebrospinal fluid (CSF) is about 0.5 per cent that of plasma. This difference in concentration is generally considered (1) to be dependent upon the blood-CSF barrier. The normal concentration gradient of protein from a low level in the ventricles, 6 to 15 mg. per cent, to an intermediate level in the cisterna magna, 15 to 25 mg. per cent, to the highest lev...

Background: Pleural effusion is a common clinical problem resulting from thoracic or systemic diseases. Several biochemical parameters, serological and cytological markers are used to classify the type of pleural effusion. The oldest classification is Light’s criteria which categorizes exudates by meeting one of these criteria: Pleural to serum protein ratio> 0.5, pleural to serum LDH ratio> 0....

1. The density-gradient distribution patterns of acid phosphatase, Trypan Blue and denatured (125)I-labelled albumin were studied by discontinuous sucrose- and isopycnic sucrose-density-gradient centrifugation on combined heavy and light mitochondrial (M+L) fractions of liver isolated from normal rats and from rats injected with Triton WR-1339. 2. The results obtained from the subfractionation ...