Hyperglycaemia induced non enzymatic glycation is accelerated in diabetic patients and aggressively involved in diabetes progression. Human serum albumin (HSA) is the most abundant protein in blood circulation. In hyperglycaemia, it undergoes fast glycation and results in the impairment of structure. Our previous study has demonstrated structural alterations in Amadori-albumin modified with dif...

We compared the fructosamine activity in sera from healthy and diabetic subjects with the degree of protein glycation detected by a liquid-chromatographic method. The latter technique measures furosine as a specific product after hydrolysis of epsilon-amino-fructose-lysine. Our results indicate that the fructosamine assay measures the extent of glycation of purified human serum albumin correctl...

background: receptor for advanced glycation end products (rage) plays a causative role in diabetes. garlic (allium sativum) belongs to compounds with anti-glycation activity that can be considered as probable therapeutic approaches in delaying or preventing the onset of diabetes complications. the aim of this study was to evaluate the influence of garlic on the rage expression and proinflammato...

Glycation is a non-enzymatic reaction between the carbonyl groups of sugars and the amino groups of proteins, nucleic acids and lipids. This process results in the formation of early glycation products, which rearrange to form more stable advanced glycation end products (AGEs). Glycation has been linked to a number of diseases such as, Alzheimer’s, diabetes mellitus, cataract, Parkinson’s, phys...

Non-enzymatic glycation is a process, which can be best described as significant posttranslational modification of various proteins. It emerges in hyperglycemic conditions and may have an impact on albumin stability well its activity physical chemical properties, essentially affecting all physiological functions. The goal this research was to answer the following questions: (i) how does defatte...

human serum albumin has been used as a model protein for protein folding and ligand binding studies over many decades. due to its long life period and high concentration in plasma, hsa is highly sensitive to glycation. it is reported that 175 mg/dl glucose concentration is a threshold of kidney activity for the beginning of excretion of glucose. ph denaturation of hsa in absence and presence of...

       In this study, we examined the antioxidant activities of methanolic extract of three endemic species of Salvia from Iran (S. lachno...