Antimicrobial susceptibility testing for Helicobacter pylori is increasingly important due to resistance to the most used antimicrobials agents. Only agar dilution method is approved by CLSI, but it is difficult to perform routinely. We evaluated the reliability of E-test and disk diffusion comparing to agar dilution method on Helicobacter pylori antimicrobial susceptibility testing. Susceptibi...

Background: A special hydroxypropylmethylcellulose powder (Nasaleze®) has been used for the alleviation of nasal symptoms of allergic rhinitis since 1994. The efficacy of the product has been recently proven but the mechanism of action was still largely unknown. The aim of the study was to investigate the hypothesis that the gel formed after moisture absorption in the nose might act as mechanic...

Previously, we showed that microbial susceptibility to antimicrobials in concentrations non-toxic for human cells in culture could be tested using the wet disc topical antimicrobial assay. In this report, wet disc assay and agar well diffusion assay results were compared testing the susceptibility of Ps. aeruginosa isolates from burn patients to concentrations of Polymyxin B non-toxic for cultu...

Korgenski and Daly recommend charcoal horse blood agar (Regan-Lowe agar) for the detection of erythromycin resistance in Bordetella pertussis by agar dilution, Etest methodology, or agar diffusion. MICs for most isolates were #0.06 to 0.12 mg/ml by agar dilution and the Etest, while the MIC for the only resistant strain was 16 to 64 mg/ml (4). Charcoal agar may be suitable for discrimination be...

Antibiotic susceptibility testing of 70 pediatric Helicobacter pylori isolates was performed by using screening agar and disk diffusion methods. Resistance to metronidazole and tinidazole was 72 to 79% and 71 to 81% by modified disk diffusion and 77% and 78% by screening agar, respectively. Susceptibilities to amoxicillin, ampicillin, clarithromycin, tetracycline, erythromycin, and ciprofloxaci...

A variety of devices such as stainless-steel or ceramic cylinders, single or multiple in design, and paper discs of several sizes are commonly used for antibiotic assays performed by the agar diffusion method. Holes cut in agar (agar wells) are wells with melted agar is often necessary to obtain uniform inhibition zones (2). This note describes a method for the preparation of agar-well plates w...

A simple, reliable, and inexpensive assay for quantitation of the imidazole drugs miconazole, RV 40,500, and RV 41,400 was tested. The assay, similar to a Kirby-Bauer test, was sensitive to less than or equal to 0.2 mug of drug per ml and linear from less than or equal to 0.2 to 10.0 mug/ml. Concentration of inoculum and agar depth in test plates was not as critical as the type of medium, amoun...