Fumonisin B₁ (FB₁) is often a co-contaminant with aflatoxin (AF) in grains and may enhance AF's carcinogenicity by acting as a cancer promoter. Calcium montmorillonite (i.e. NovaSil, NS) is a possible dietary intervention to help decrease chronic aflatoxin exposure where populations are at risk. Previous studies show that an oral dose of NS clay was able to reduce AF exposure in a Ghanaian popu...

the effects of aflatoxin (af) (0.0 and 0.5m g kg-1) and high grade sodium bentonite (hgsb) (5, 7.5 and 10 g kg-1) were tested in an in vivo study including 8 dietary treatments with three replicates of 14 birds per diet per replicate, using a total of 336 broiler chicks up to five weeks. results showed that chicks receiving af contaminated feed had significantly (p≤ 0.05) suppressed body weight...

The mycological profile of the retail wheat flour selling in different markets at Jeddah (Kingdom of Saudi Arabia) was studied. The most common genera were Aspergillus (isolated from 70% of the tested samples), Penicillium (30%), Eurotium (14%), and in a lesser extent Fusarium (20%) and Alternaria (18%). Twenty-nine strains of Aspergillus flavus were screened for their ability to produce aflato...

The development of molecular dosimetry methods will simplify the identification of people at high risk for cancer. A combined monoclonal antibody ¡mmunoaffinitychromatography/high performance liquid chromatography method has been devised to isolate and quantify aflatoxinDNA adducts and other metabolites in rat urine samples. We report the production of 11 different monoclonal antibodies recogn...

Objective: Due to the importance of poultry production, purpose study are show efficacy Tomatopowderin alleviating deleterious effects aflatoxin in broilers and modulating oxidative stress. Methods:One hundred one-day-old fattening chicks (Ross 308) were used present work which lasted for 35 days administration 28 days. In control condition, birds randomly distributed into 5 groups, 20 each gro...

Lysine methyltransferases transfer methyl groups in specific lysine sites, which regulates a variety of important biological processes in eukaryotes. In this study, we characterized a novel homolog of the yeast methyltransferase DOT1 in A. flavus, and observed the roles of dot1 in A. flavus. Deletion of dot1 showed a significant decrease in conidiation, but an increase in sclerotia formation. A...

Cytotoxicity testing of aflatoxin (AF) on the viability of cells grown in cultures can be widely used to predict the potential toxic effects of AF in animals. To this end, an in vitroexperimental study was conducted to ascertain the toxic effects of AF extracts obtained from compound feeds in South Africa on human lymphocytes in comparison to that of an AFB1 standard. The approach adopted was o...

Since 2003, non-compliant aflatoxin concentrations have been detected in maize produced in Italy. The most successful worldwide experiments in aflatoxin prevention resulted from distribution of atoxigenic strains of Aspergillusflavus to displace aflatoxin-producers during crop development. The displacement results in lower aflatoxin concentrations in harvested grain. The current study evaluated...

This study was aimed to determine the effects of the glucomannan added to aflatoxin- (AF-) contaminated diet on the sacculus rotundus and peripheral blood lymphocytes of New Zealand rabbits by histological and enzyme histochemical methods. Twenty-four adult rabbits of both sexes were divided into four equal groups, namely, as control, glucomannan 0.2 g/day, AF 125 μg/kg/day, and glucomannan com...

The covalent binding of [3H]aflatoxin B1 (AF) to liver DNA was determined, 6 h after oral administration to male rabbits. A Covalent Binding Index, CBI (mumol AF/mol DNA-P)/(mmol AF/kg b.w.) = 8,500 was found. Pretreatment of rabbits with AF coupled to bovine serum albumin in Freund's adjuvant led to the production of AF-directed antibodies. Administration of [3H]AF to such immunized rabbits re...