AIM To evaluate the effect of dentine conditioning on migration, adhesion and differentiation of dental pulp stem cells. METHODOLOGY Dentine discs prepared from extracted human molars were pre-treated with EDTA (10%), NaOCl (5.25%) or H2 O. Migration of dental pulp stem cells towards pre-treated dentine after 24 and 48 h was assessed in a modified Boyden chamber assay. Cell adhesion was evalu...

OBJECTIVES This study examined the changes in hydraulic conductance and ultrastructure of dentine bonded with simplified etch-and-rinse adhesives to oxalate desensitiser pre-treated acid-etched dentine. METHODS Human dentine disks were acid-etched, treated with an oxalate desensitiser (BisBlock, Bisco), and bonded with One-Step (OS), Single Bond (SB), OptiBond Solo Plus (OB) or Prime and Bond...

A better understanding of the microstructure and mechanical properties enamel dentine may enable practitioners to apply current adhesive dentistry protocols clinical cases involving disorders (dentinogenesis imperfecta or dysplasia). Publications (up June 2020) investigating were browsed in a systematic search using PubMed/Medline, Embase Cochrane Library electronic databases. Two authors indep...

This study evaluated the effectiveness of carboxymethyl cellulose (CMC) hydrogel as a dental gel in delivering hydroxyapatite (HAp) to dentine tubules and reducing/eliminating dental hypersensitivity. The hydrogel was prepared by mixing solutions of CMC/ glycerol and distilled water/sorbitol then modified to contain 0%, 10%, 20% and 30% HAp. The pH of the hydrogels decreased and viscosity incre...

OBJECTIVES Amalgam restorations are replaced by adhesively placed composite resin restorations at an increasing rate. After the removal of amalgam dentine often shows marked dark discoloration that is attributed to the penetration of corrosion products from overlying amalgams. It is questioned whether penetration of metals into dentine affects the dentine as a substrate for adhesive procedures....

We investigate the nonlinear properties of dentine from the upper canines of coyotes Canis latrans with bending tests. With the results we predict the behavior of whole canines under load. Coyote dentine is not homogeneous but is stronger and more ductile farther away from the pulp cavity. The modulus of rupture (MOR) first increases and then declines with distance from the pulp cavity. Our ana...

The purpose of this in vitro study was to investigate the effect of 1% NaOCl (15 min) and MTAD on dentine shear bond strength, when using the Scotchbond XT(3M ESPE) adhesive system. Thirty extracted, human, third molar teeth were embedded into metal rings with self-curing acrylic resin. The teeth were ground wet using 600-grit silicone-carbide paper to expose superficial dentine. The samples we...

Calcium hydroxide's anti-bacterial action relies on high pH. The aim here was to investigate hydroxyl ion diffusion through dentine under different conditions. Teeth were divided into control (n = 4) and four experimental groups (n = 10): Group 1-no medicament; Group 2-Calmix; Group 3-Calmix/Ledermix; Group 4-Calasept Plus/Ledermix; Group 5-Pulpdent/smear layer. Deep (inner dentine) and shallow...

In the developing tooth, 1-hydroxyethylidene-1,1-bisphosphonate (HEBP) causes hypoplasia and hypomineralization of enamel and dentine. The present study was undertaken to clarify the effects of HEBP on the formation of dental tissues of tooth germs in an organ culture system. Mandibular first molars from 17.5-day-old mouse embryos were cultured with or without 250 microM HEBP in culture medium....