the purpose of the present study is to investigate the cytotoxicity/apoptotic effect of 2-chloro-2′-deoxyadenosine, cladribine, (2-cda) in the human breast cancer cell line, mda-mb468 (estrogen receptor negative, er−). mtt [3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2h tetrazolium bromide] assay, annexin v-fluorescein/pi and hoechst 33258 staining were used to detect cytotoxicity and cell apopto...

adenosine acts as neuromodulator in the brain, which its involvement in a wide range of brain processes and diseases has been studied, such as epilepsy, sleep, anxiety, panic disorder, alzheimer’s disease, parkinson’s disease and schizophrenia. adenosine receptors have been detected: a1r, a2ar (a2ar), a2br, and a3r. a1r and a2r inhibit camp production, while a2ar and a2br stimulate camp product...

The pregnane X receptor (PXR) is a master in regulating the metabolism and transport of structurally diverse endogenous compounds. Activation PXR by xenobiotics potentially induces adverse effects and...

background: phytoestrogens, a group of plant-derived polyphenolic compounds have recently come into considerable attention due to the increasing information on their potential adverse effects in human health. some of phytoestrogens show estrogenic activity that may be carcinogenic for human. in the present study, we investigated the transcriptional effects of variety of phytoestrogens on the bo...

Objective(s): This study aims to investigate the pathogenicity and possible mechanisms of S100A9 function in mice models of scleroderma. Materials and Methods: The content of S100A9 in the skin tissues of mice with scleroderma was determined. Different concentrations of bleomycin (BLM) and S100A9 were subcutaneously injected into the backs of mice simultaneously, and then pathological changes i...

Central ossifying fibroma is a benign, slow-growing tumor of mesenchymal origin with a predilection for the mandibular premolar and molar areas. The immunophenotype of T cells involved in the antitumor response against this benign tumor is unknown. In this case report, we described a case of a 48-year-old woman presenting with a very large recurrent ossifying fibroma in the mandible, which was ...

the effect of epinephrine on phosphatidate phosphohydrolase (pap) activity of isolated human hepatocytes was studied. epineprine inhibited the enzyme activity progressively at concentrations above 0.1 µm, reaching a maximum inhibition of 64.5% at 100 µm concentration. inclusion of alprenolol, a β-receptor blocker, in the incubation mixture abolished the inhibitory effect of epinephrine on pap, ...

Tumor necrosis factor receptor-associated factors (TRAFs) play vital roles in tumor receptor (TNF-R) and interleukin-1 receptor/Toll-like (IL-1R/TLR) mediated signaling pathway. However, the role that TRAF7 plays host immune responses is largely unknown comparison to extensive in-depth research has been conducted on other members of TRAF family. Notably, Lc -TRAF7, a cloned ortholog, was discov...

ige-mediated cell signaling, induced by cross-linking of high affinity receptor for ige (fcεri) in the presence of antigen (ag), is a well known mechanism described for mast cell activation in allergy and hypersensitivity reactions, which induces a spectrum of cellular responses such as secretion and up-regulation of cell surface fcεri. although for several years  ige  binding  to  fcεri  was  ...

The effect of epinephrine on phosphatidate phosphohydrolase (PAP) activity of isolated human hepatocytes was studied. Epineprine inhibited the enzyme activity progressively at concentrations above 0.1 µM, reaching a maximum inhibition of 64.5% at 100 µM concentration. Inclusion of alprenolol, a β-receptor blocker, in the incubation mixture abolished the inhibitory effect of epinephrine on P...