Specimens of cells derived from tumors of the human female genital tract plus normal cells as standards have been divided into aliquots and stained according to acridine orange or pararosanilin:Feulgen procedures. Acridine orange-stained cells were slit-scanned for 535 nm nuclear fluorescence; Feulgen-stained cells were comb-scanned for 580 nm nuclear absorbance. For each specimen examined, the...

INTRODUCTION: Micronuclei (MN) are microscopically visible round or oval cytoplasmic chromatin masses in the extra nuclear vicinity, originating from aberrant mitosis, chromatic fragments or whole chromosomes. The local factors like smoking exerts an influence on oral mucosa cells which are reflected as cellular changes resulting in the formation of MN. AIM: To evaluate and compare the MN index...

Nucleic acids can undergo dynamic conformational changes associated with the regulation of biological processes. A molecule presenting larger affinities for alternative structures relative to a duplex is expected to modify such conformational equilibria. We have previously reported that macrocyclic bis-acridine binds preferentially to single-stranded regions, especially DNA hairpins, due to ste...

The acridine ring system and the benzene ring in the title compound, C(20)H(13)NO(2), are oriented at a dihedral angle of 6.4 (2)°. The carboxyl group is twisted at an angle of 83.6 (2)° relative to the acridine skeleton. The mol-ecules in the crystal are arranged in stacks along the b axis, with two of the acridine rings involved in multiple π-π inter-actions [centroid-centroid distances in th...

Borreliacidal antibody has been shown to be important for the serodiagnosis of Lyme disease and determination of immune status. Our results show that borreliacidal antibody can be rapidly and accurately detected by flow cytometry. Acridine orange was added to normal and immune sera containing Borrelia burgdorferi organisms in the presence and absence of complement prior to data acquisition by f...

The recent paper by Rotimi and colleagues 1 does not mention the acridine orange stain 2 when comparing staining methods for the identification of Helicobacter pylori. The acri-dine orange stain uses ultraviolet fluores-cence in the identification of bacteria. The typical curved morphology of H pylori can easily be diVerentiated from other bacteria. I have used this quick, cheap, and reliable s...

Acridine orange decreases the amplitude of the 295-nm circular dichroism (CD) trough of ribosomal ribonucleic acid (rRNA) where the trough has been related to coil character. Since acridine orange is known from earlier work to intercalate between base pairs of nucleic acids, causing an unwinding of the coil, and our studies show a decrease in the 295-nm CD trough, it appears that CD measurement...

An analytical scheme developed for determination of PAH and aminoazaarenes has been applied to the analysis of aza-PAH formed in thermally treated high-protein food. The clean-up procedure, which was based on tandem solid-phase extraction (SPE) on columns filled with Extrelut diatomaceous earth and with cation exchanger (propylsulphonic acid, PRS), enabled selective isolation of carcinogenic az...

In the crystal structure of the title compound, C(21)H(15)BrNO(2) (+)·CF(3)SO(3) (-), the cations form inversion dimers through π-π inter-actions between the acridine ring systems. These dimers are further linked by C-H⋯π and C-Br⋯π inter-actions. The cations and anions are connected by multidirectional C-H⋯O and C-F⋯π inter-actions. The acridine and benzene ring systems are oriented at 10.8 (1...

In the crystal structure of the title compound, C(22)H(18)NO(2) (+)·CF(3)SO(3) (-), adjacent cations are linked through C-H⋯π and π-π inter-actions, and the cations and anions are connected by C-H⋯O and C-F⋯π inter-actions. The acridine and benzene ring systems are oriented at a dihedral angle of 3.0 (1)°. The carboxyl group is twisted at an angle of 83.1 (1)° relative to the acridine skeleton....