Purification of pea (Pisum sativum) seedling NAD kinase by DEAE-cellulose column chromatography resulted in loss of activity, due to dissociation of an activator from the enzyme. The purified enzyme preparation, which was almost completely inactive, regained the activity when the activator was added back.The activator was purified 320-fold by ion exchange chromatographies. The activator was sus...

tenecteplase is a variant of tissue plasminogen activator (t-pa) which has better pharmacokinetic properties and more selective thrombolytic activity. in the present study, we describe a rapid method to introduce three sets of mutation into defined positions in t-pa cdna by a site-directed mutagenesis based on a megaprimer pcr approach to produce tenecteplase coding sequence where amino acids a...

standard protocols should be established for treating eligible stroke patients with tissue plasminogen activator (tpa) (recommendation class i, level of evidence b). the iranian standard protocol of intravenous thrombolysis with recombinant tissue plasminogen activator (ivttpa) is the best possible and easy to use method for performing intravenous thrombolysis in iran. this protocol overcomes p...

The Cover Feature represents the concept of employing two (frustrated) Lewis acid-base pairs within a pincer-type compound to activate defiant N2-molecule; N≡N triple bond is thereby extended by remarkable 0.34 Å. Once caught tailor-made activator molecule, N2 prone stepwise reduction with hydride (compensated protons) finally release one molecule ammonia, whereas remaining nitrogen could not b...

Abstract The construction material from anhydrite-II phosphogypsum is dense in structure and has good water resistance. But its disadvantages are low hydration activity long setting time. In this work, modified by adding a composite activator, which made of sulfuric acid steel slag, β-hemihydrate gypsum calcium aluminate cement. With this, the rate clearly increased time shortened. performance ...

it has been shown that geopolymerization can transform a wide range of waste aluminosilicate materials into building materials with excellent chemical and physical properties such as fire and acid resistance. in this research work, geopolymerization of construction waste materials with different alkali-activators based on combinations of na2sio3 and naoh has been investigated. a number of syste...

In the transcriptional response of Saccharomyces cerevisiae to stress, both activators and repressors are implicated. Here we demonstrate that the ion homeostasis determinant, HAL1, is regulated by two antagonistically operating bZIP transcription factors, the Sko1p repressor and the Gcn4p activator. A single CRE-like sequence (CRE(HAL1)) at position -222 to -215 with the palindromic core seque...