BACKGROUND A number of adhesion molecules are involved in the process of neutrophil infiltration into the lung. P-selectin is one of these neutrophil-endothelial cell adhesion molecules. A study was undertaken to examine the involvement of P-selectin in the development of bleomycin induced inflammation and the ability of N-acetyl-L-cysteine to reduce the potential expression of this selectin in...

Novel SF5-bearing maleimide and acrylamide derivatives were synthesised as potential [18F]radio-prosthetic groups for radiolabelling peptides proteins. The efficacy of selected prosthetic was first assessed through bioconjugation with protected model amino acid derivatives. These reactions investigated on an analytical scale via LC-MS across a pH range to quantitatively evaluate this group’s re...

n-acetyl cysteine (nac), as a nutritional supplement, is a greatly applied antioxidant in vivo and in vitro. nac is a precursor of l-cysteine that results in glutathione elevation biosynthesis. it acts directly as a scavenger of free radicals, especially oxygen radicals. nac is a powerful antioxidant. it is also recommended as a potential treatment option for different disorders resulted from g...

The measurement of metabolites constitutes a useful tool for detection of exposure and in pharmacokinetic studies. Epoxidation with subsequent glutathione conjugation and mercapturic acid formation is an important deactivation pathway for naphthalene, a toxin which presumably causes lung disease. The mercapturic acid conjugates of naphthalene [NaphMA (1), N-acetyl-S-(1,2-dihydro-1-hydroxy-2-nap...

The inhibition of Salmonella typhimurium by 1,2,4-triazole appears to be mediated through an effect on L-cysteine biosynthesis. O-Acetylserine sulfhydrylase A, the final enzyme in the L-cysteine biosynthetic pathway, was found to catalyze a reaction (triazolylase) between O-acetyl-L-serine and 1,2,4-triazole, giving 1,2,4-triazole-1-alanine as a product. In wild type S. typhimurium grown on 4 m...

Although SAT (serine acetyltransferase) of Escherichia coli, which catalyses the first step in cysteine synthesis, proceeds via a random-order ternary complex reaction mechanism [Hindson and Shaw (2003) Biochemistry 42, 3113-3119], it has been suggested that the nearly identical enzyme from Salmonella typhimurium might involve an acetyl-enzyme intermediate [Leu and Cook (1994) Protein Peptide L...

Overdose of acetaminophen causes severe hepatic necrosis in humans and experimental animals. Studies on its hepatotoxicity remain a very active area since some of current data are still uncertain. In this study, freshly isolated rat hepatocytes were used to determine the effects of garlic extract and its component, allicin on the acetaminophen-induced cell cytotoxicity and to compare with the e...

PURPOSE: To investigate the effect of strenuous exercise and antioxidant administration on proand antiapoptotic protein expression in intestinal lymphocytes. METHODS: Female C57BL/6 mice (N = 52) were randomly assigned to receive N-acetyl cysteine (NAC; 1 g.kg(-1)) or saline (SAL) 30 min before treadmill exercise (EX) for 90 min and 2 degrees slope (30 min at 22 m.min(-1), 30 min at 25 m.min(-1...