We have developed a procedure for immune selection in an established human lymphoid cell line based on HL-A, the major human histocompatibility locus. After a single brief exposure to selective conditions, HL-A2 variant clones were isolated from an HL-A2/HL-A3 heterozygous line. The variant clones occurred at a frequency of about 1 x 10(-6). The variant phenotype was stable during prolonged gro...

The dominant trans-acting major histocompatibility complex (MHC)-linked class I modifier (cim) locus, previously recognized through its ability to determine altered alloantigenicity of a rat class I molecule, RT1.A3, is shown here to influence class I intracellular transport. The MHC recombinant laboratory rat strains PVG.R1 and PVG.R8 display unusually long retention of RT1.Aa within the endop...

Source and Description: Lambda clone 135 derived from a YAC from the CEPH library, mapping to an interval containing at least part of the Kallmann locus (1). DNA sequences flanking a (C-A)17 repeat of the lambda clone were used to design the polymerase chain reaction (PCR) primers. The polymorphism can be typed using PCR as previously described (2). Polymorphism: Five allelic fragments were res...

This deliverable summarizes the results and experiences of working group A3 on establishing personalization strategies in the Semantic Web by presenting best practices for personal information systems in the Semantic Web. It builds on the work reported in Deliverables A3-D1 to A3-D9 which have investigated on necessary theoretical foundations (Deliverables A3-D1, A3D4 and A3-D7), Prototypes and...

Thirteen novel avipoxviruses were isolated from birds from different regions of South Africa. These viruses could be divided into six groups, according to gross pathology and pock appearance on chick chorioallantoic membranes (CAMs). Histopathology revealed distinct differences in epidermal and mesodermal cell proliferation, as well as immune cell infiltration, caused by the different avipoxvir...

Aim. The aim of the study was to analyze diversity and frequencies alleles at minor gliadin locus Gli-A3 in groups common winter wheat cultivars developed different breeding institutions Ukraine periods time. Methods. Acid polyacrylamide gel electrophoresis gliadins carried out. To identify allele cultivar Myronivska storichna, segregation a sample F2 seeds from crossing with Bezosta 1 analyzed...