Mitosis is thought to be triggered by the activation of Cdk-cyclin complexes. Here we have used RNA interference (RNAi) to assess the roles of three mitotic cyclins, cyclins A2, B1, and B2, in the regulation of centrosome separation and nuclear-envelope breakdown (NEB) in HeLa cells. We found that the timing of NEB was affected very little by knocking down cyclins B1 and B2 alone or in combinat...

Based on agricultural biomass resources and utilization in Shaanxi province, this experiment used decomposing mushroom residues, pine needles, peanut shells compounded with river sand as organic cultivation substrates, compared garden soil, carried out pot experiments to study the effect of different soilless substrates tomato yield, size number. The results show that CK1 (garden soil) CK2 (dec...

The human-health risk posed by gardening near a legacy mine and smelter in an arsenic-endemic region of Arizona was characterized in this study. Residential soils were used in a greenhouse study to grow common vegetables, and local residents, after training, collected soil, water, and vegetables samples from their home gardens. Concentrations of arsenic measured in water, soil, and vegetable sa...

this study was conducted in split plot design based on a randomized complete block design in naghadeh region, urmia, iran in 2009-10. two sowing patterns including a1) single row planting with 50 cm between-row spacing, and a2) two rows planting on the hill with 40 cm distance and 50 cm between-row spacing (40×50) were allocated to main plots and four furrow compactions including b1) control, b...

Let X be an n–element finite set, 0 < k < n/2 an integer. Suppose that {A1, B1} and {A2, B2} are pairs of disjoint k-element subsets of X (that is, |A1| = |B1| = |A2| = |B2| = k,A1 ∩ B1 = ∅, A2 ∩ B2 = ∅). Define the distance of these pairs by d({A1, B1}, {A2, B2}) = min{|A1−A2|+ |B1−B2|, |A1−B2|+ |B1−A2|}. It is known ([2]) that this is really a distance on the space of such pairs and that the ...

Heterogeneous nuclear ribonucleoprotein (hnRNP) A2/B1 is an RNA binding protein that is required for maturation of mRNA precursor. Tockman et al. previously reported that hnRNP A2/B1 with a M(r) of 31,000 is overexpressed from the early clinical stage of human lung cancer (M. S. Tockman et al., J. Clin. Oncol., 6: 1685-1693, 1988). However, when hnRNP A2/B1 mRNA and hnRNP B1 mRNA were separatel...

Soil carbon and nitrogen contents play an important role in sustaining soil physical and chemical quality and help to have healthy environments. The continues conversion of rangelands to arable lands has the potential to change carbon and nitrogen sequestration. In this study to evaluate the effects of land use change on soil organic carbon and nitrogen stock, forty samples collected from north...

BACKGROUND & OBJECTIVE The differentially expressed nuclear matrix proteins have great effects on canceration and regulation of cell differentiation. This study was to explore the existence and distribution of ribonucleoprotein hnRNP A2/B1 in nuclear matrix and its co-localization with Actin and Prohibitin in human osteosarcoma MG-63 cells before and after hexamethylene bisacetamide (HMBA) trea...

Recent reports have demostrated a link between expression of members of the family of heterogeneous nuclear ribonucleoproteins (hnRNPs) and cancer. Overexpression of hnRNP A2/B1 correlated with the eventual development of lung cancer in three different clinical cohorts. We have studied the expression of hnRNP A2/B1 messenger RNA (mRNA) and protein during mammalian development. The expression of...

The monoclonal antibody 703D4, which binds heterogeneous nuclear ribonucleoprotein A2/B1 (hnRNP A2/B1), has been reported to detect lung cancer more than a year earlier than routine chest X-ray or cytomorphology. To explore the biological basis of this detection, we studied the expression of this antigen in the central airways of smokers with evidence of bronchial metaplasia using specimens fro...