The antibody response to various combined polyvalent Pasteurella haemolytica vaccines was studied in sheep and cattle. In sheep, certain oil adjuvant vaccines gave rise to a better antibody response to P. haemolytica than an A1(OH)3-adsorbed vaccine. This finding, however, was not consistent for all serotypes, and with respect to P. multocida, oil adjuvants had no advantage. Furthermore, it was...

Hypertonicity increases urea transport, as well as the phosphorylation and membrane accumulation of UT-A1, the transporter responsible for urea permeability in the inner medullary collect duct (IMCD). Hypertonicity stimulates urea transport through PKC-mediated phosphorylation. To determine whether PKC phosphorylates UT-A1, eight potential PKC phosphorylation sites were individually replaced wi...

An experimental model of pneumonic pasteurellosis in sheep was used to investigate the role of serum antibody in resistance to this disease. Lambs which had been vaccinated with a sodium salicylate extract of Pasteurella haemolytica type A1 were protected against challenge with PI3 virus followed by P. haemolytica type A1 7 days later. The majority of untreated lambs and lambs which had receive...

BACKGROUND AND METHODS Monoclonal antibodies (mAb) specific for the oligosaccharide epitopes of glycoproteins or glycolipids, such as blood group antigens, are powerful tools for studying the antigenic structure of normal and pathological cells and tissues. Anti-A human red blood cell monoclonal antibodies were produced by immunizing mice with normal cells, but only a few fulfilled the conditio...

The heterogeneous nuclear ribonucleoprotein A1 (hnRNP A1) functions in the packaging of nascent RNA polymerase II transcripts and participates in a variety of nuclear and cytoplasmic processes that modulate gene expression. The RNA binding characteristics of hnRNP A1 suggest that it can modulate the expression of specific genes, but little is known about its possible targets in vivo. In this ar...

Terminal B-cell differentiation is a multi-step process, from short-lived plasmablasts to mature long-lived plasma cells (PC). The anti-apoptotic Bcl-2 family member Bfl-1/A1 plays a critical role in the survival of mature B cells. However, its potential involvement at the later stages of B-cell development remains highly controversial. Our aim was thus to clarify the place of Bfl-1/A1 in the b...

Botulinum neurotoxins (BoNTs) are extremely potent toxins that are capable of causing death or respiratory failure leading to long-term intensive care. Treatment includes serotype-specific antitoxins, which must be administered early in the course of the intoxication. Rapidly determining human exposure to BoNT is an important public health goal. In previous work, our laboratory focused on devel...

Factor VIIIa, the protein cofactor for factor IXa, is comprised of A1, A2, and A3-C1-C2 subunits. Recently, we showed that isolated A2 subunit enhanced the kcat for factor IXa-catalyzed activation of factor X by approximately 100-fold ( approximately 1 min-1), whereas isolated A1 or A3-C1-C2 subunits showed no effect on this rate (Fay, P. J., and Koshibu, K. J. (1998) J. Biol. Chem. 273, 19049-...

A group A1 diabetic received a pancreas-spleen transplant from a group 0 donor. Severe immune hemolysis due to anti-A ensued, requiring graft splenectomy. The transplanted spleen can be a potent source of blood group antibody.