While a functional quorum sensing system has been identified in the acidophilic chemolithoautotrophic Acidithiobacillus ferrooxidans ATCC 23270(T) and shown to modulate cell adhesion to solid substrates, nothing is known about the genes it regulates. To address the question of how quorum sensing controls biofilm formation in A. ferrooxidans (T), the transcriptome of this organism in conditions ...

Rate equations and kinetic parameters were obtained for various reactions involved in the bacterial oxidation of pyrite. The rate constants were 3.5 muM Fe per min per FeS(2) percent pulp density for the spontaneous pyrite dissolution, 10 muM Fe per min per mM Fe for the indirect leaching with Fe, 90 muM O(2) per min per mg of wet cells per ml for the Thiobacillus ferrooxidans oxidation of wash...

The chromosomal arsenic resistance genes of the acidophilic, chemolithoautotrophic, biomining bacterium Thiobacillus ferrooxidans were cloned and sequenced. Homologues of four arsenic resistance genes, arsB, arsC, arsH, and a putative arsR gene, were identified. The T. ferrooxidans arsB (arsenite export) and arsC (arsenate reductase) gene products were functional when they were cloned in an Esc...

Applicability of the manometric method for studying the oxidation of pyritic material in the presence of bacteria has been demonstrated. Resting cells of Ferrobacillus ferrooxidans accelerated the oxidation of coal pyrites and coarsely crystalline marcasite, but were inactive on coarsely crystalline pyrite. Resting cells of Thiobacillus thiooxidans were inactive on all pyrites tested. Oxidation...

this study deals with bioleaching of the low grade uranium ore of saghand mine in iran using a mixed culture of mesophilic bacteria (acidithiobacillus ferrooxidans, acidithiobacillus thiooxidans and leptospirillum ferrooxidans). experiments were carried out in shaking flask examining the effect of temperature, initial ferrous ion concentration, pulp density and particle size. variations of ph, ...

The synthesis, molecular structure, and electronic structure characterization of two five-coordinate high-spin imidazolate-ligated iron(II) porphyrinates are reported. Their electronic structure, as deduced from Mössbauer spectra obtained in strong magnetic fields, is distinctly different from that of the analogous imidazole-ligated species. The resulting electronic structure models are consist...

Ferrobacillus ferrooxidans was isolated and named by Leathen and Braley (1) in 1954 and reported to be an obligate chemoautotroph which utilizes the oxidation of inorganic ferrous iron as a primary energy source. A somewhat similar bacterium, called Thiobaeillus ferrooxiduns, had previously been isolated by Cohner, Temple, and Hinkle (2) and characterized as an obligate chemoautotroph which wou...

A combination of EXAFS, transmission electron microscopy and energy-dispersive X-ray was used to conduct a molecular and atomic analysis of the uranium complexes formed by Acidithiobacillus ferrooxidans. The results demonstrate that this bacterium accumulates uranium as phosphate compounds. We suggest that at toxic levels when the uranium enters the bacterial cells, A. ferrooxidans can detoxify...

Glucose-6-phosphate dehydrogenase was partially purified from both glucose-grown and iron-glucose-grown Thiobacillus ferrooxidans. The enzyme possesses a dual nucleotide specificity for either nicotinamide adenine dinucleotide phosphate (NADP) or nicotinamide adenine dinucleotide (NAD) and has a molecular weight of 110,000 as determined by gel electrophoresis. Evidence is presented that T. ferr...

A simple metabolic model of ferrous oxidizing bacteria based on biochemically structured balances of ATP and NAD(P)H is proposed in order to calculate maximum yield and maintenance on ATP. Similar values of growth yield and maintenance were obtained using data of ferrous iron and/or oxygen consumption in Acidithiobacillus ferrooxidans cultures on iron under different conditions. When pyrite was...