Sirtuins are protein deacetylases regulating metabolism and stress responses. The seven human Sirtuins (Sirt1-7) are attractive drug targets, but Sirtuin inhibition mechanisms are mostly unidentified. We report the molecular mechanism of Sirtuin inhibition by 6-chloro-2,3,4,9-tetrahydro-1H-carbazole-1-carboxamide (Ex-527). Inhibitor binding to potently inhibited Sirt1 and Thermotoga maritima Si...

Two different methods have been used to fatigue test four bone cements. Each method has been used previously, but the results have not been compared. The ISO 527-based method tests a minimum of 10 samples over a single stress range in tension only and uses Weibull analysis to calculate the median number of cycles to failure and the Weibull modulus. The ASTM F2118 test regime uses fewer specimen...

This study is the first study that provides useful guidelines to clinical microbiologists and technicians on the usefulness of full 16S rRNA sequencing, 5'-end 527-bp 16S rRNA sequencing and the existing MicroSeq full and 500 16S rDNA bacterial identification system (MicroSeq, Perkin-Elmer Applied Biosystems Division, Foster City, California, USA) databases for the identification of all existin...

A estratégia teórica de articular as reflexões sobre a Filosofia do Reconhecimento à Teoria da Constituição é um dos pilares fundamentais novo ramo Direito denominado Constitucional Reconhecimento. O trabalho investiga o voto ministro Barroso na ADPF 527 com base em uma que aposta relação complementaridade entre teorias dialógicas e métodos interseccionais luz possuem substrato conceitual comum...

Accumulation of vitronectin protein increased in the conditioned medium of mouse neuroblastoma Neuro2a cells during retinoic acid-induced differentiation. To study the regulatory mechanism of the increase in vitronectin expression during the differentiation, the activity of the -527/+95 vitronectin promoter was observed in Neuro2a cells with or without retinoic acid treatment. The result showed...

Src and Src-family protein-tyrosine kinases are regulatory proteins that play key roles in cell differentiation, motility, proliferation, and survival. The initially described phosphorylation sites of Src include an activating phosphotyrosine 416 that results from autophosphorylation, and an inhibiting phosphotyrosine 527 that results from phosphorylation by C-terminal Src kinase (Csk) and Csk ...

Background: Female genital schistosomiasis (FGS) can occur in S. haematobium infection and is caused by egg deposition the tract. Confirming a diagnosis of FGS challenging due to lack diagnostic reference standard. A 2010 expert-led consensus meeting proposed visual inspection cervicovaginal mucosa as an adequate standard for diagnosis. The a...

[This corrects the article on p. 527 in vol. 8, PMID: 28496419.].