How single-chain urokinase (ScuPA) mediates angiogenesis is incompletely understood. ScuPA (≥4 nM) induces phosphorylated (p)ERK1/2 (MAPK44 and MAPK42) and pAkt (Ser(473)) in umbilical vein and dermal microvascular endothelial cells. Activation of pERK1/2 by ScuPA is blocked by PD-98059 or U-0126, and pAkt (Ser(473)) activation is inhibited by wortmannin or LY-294002. ScuPA (32 nM) or protease-...

The Akt and mitogen-activated protein kinase (MAPK) pathways have been implicated in tumor cell survival and contribute to radiation resistance. However, the molecular basis for link between MAPK and Akt in cell survival response to radiation is unclear. Here, we show that c-Src-Rac1-p38 MAPK pathway signals Akt activation and cell survival in response to radiation. Ionizing radiation triggered...

Major depressive disorder (MDD) is a debilitating disease affecting a wide cross section of people around the world. The current therapy for depression is less than adequate and there is a considerable unmet need for more efficacious treatment. Dopamine has been shown to play a significant role in depression including production of anhedonia which has been one of the untreated symptoms in MDD. ...

CPT-11 [7-ethyl-10-[4-(1-piperidino)-1-piperidino] carbonyloxycamptothecin or Irinotecan] is a carbamate prodrug that is activated in vivo by carboxylesterase (CES)-2 to SN-38 (7-ethyl-10-hydroxycamptothecin), a potent topoisomerase I inhibitor. There is high interindividual variation when CPT-11 is used in the treatment of colorectal cancer. Several splice variants of CES2 are reported in the ...

The transformation behaviors on metamagnetic shape memory Ni46.4Mn38.8In12.8Co2.0 film were investigated by X-ray diffraction experiments in the temperature up to 473 K and magnetic fields μ0H up to 5 T. The prepared film showed the parent phase with L21 structure at 473 K, and with preferred orientation along the 111 plane. The magnetic field induced reverse transformation was directly observe...

Historical Background 461 Types of Insulin 461 Rapid-Acting Insulins 461 Intermediate-Acting Insulins 462 Long-Acting Insulins 462 Insulin Purity, Species, and Concentration 463 Beef and Pork Insulins 463 Human Insulin 463 Insulin Concentrations 464 Initiation of Insulin Therapy 464 Coals of Therapy 465 Treatment Strategies 466 Single-Dose Regimens 466 Twice-Daily Regimens 467 Multiple Daily In...

Elevated hepatic expression of fatty acid elongase-5 (Elovl5) induces FoxO1 phosphorylation, lowers FoxO1 nuclear content, and suppresses expression of genes involved in gluconeogenesis (GNG). In this report, we define the molecular and metabolic basis of Elovl5 control of FoxO1 phosphorylation. Adenoviral-mediated (Ad-Elovl5) induction of hepatic Elovl5 in diet-induced obese, glucose-intoleran...