Rabbit antibodies raised against the major isozymes of cytochrome P-450 isolated from hepatic microsomes of beta-naphthoflavone- (BNF) and phenobarbital-treated rats (cytochrome P-450 BNF-B2 and cytochrome P-450 PB-B2, respectively) and against rat liver NADPH-cytochrome P-450 reductase were used to localize these enzymes immunohistochemically in the rat ventral prostate. Using the unlabeled an...

Is it possible to distinguish algebraic from transcendental real numbers by considering the b-ary expansion in some base b 2? In 1950, É. Borel suggested that the answer is no and that for any real irrational algebraic number x and for any base g 2, the g-ary expansion of x should satisfy some of the laws that are shared by almost all numbers. For instance, the frequency where a given finite se...

The debrisoquine/sparteine-type polymorphism of drug oxidation presumably is caused by the absence or deficiency of cytochrome P-450 (P-450) isozyme(s). Using bufuralol 1‘-hydroxylation as a prototype reaction of this polymorphism, two functionally distinct forms, P-450 buf I and P-450 buf 11, with identical apparent M, of 50,000 were purified from liver microsomes of three different human live...

The 7a-hydroxylation of taurodeoxycholic acid and the 6&hydroxylation of taurochenodeoxycholic acid and lithocholic acid were studied with a reconstituted system from rat liver microsomes consisting of partially purified cytochrome P-450, NADPH-cytochrome P-450 reductase, a synthetic phosphatidylcholine and NADPH or an NADPH-generating system. 7a-Hydroxylase activity was observed with cytochrom...