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We report Zeeman infra-red spectroscopy of electronic-nuclear levels 5I8 →5I7 transitions Ho3+ in the C4v(F−) centre CaF2 with magnetic field along ⟨111⟩ direction crystal. Transitions to lowest 5I7 state, an isolated electronic doublet, and next group states, a pseudo-quadruplet consisting doublet two nearby singlets, exhibit strongly non-linear splittings intensity variations. Simulated spect...
Protein A chromatography is widely used as a capture step in monoclonal antibody (mAb) purification processes. Antibodies and Fc fusion proteins can be efficiently purified from the majority of other complex components in harvested cell culture fluid (HCCF). Protein A chromatography is also capable of removing modest levels of viruses and is often validated for viral clearance. Historical data ...
The membrane receptor for retinol-binding protein (RBP) has been solubilized from human placental brush-border membranes with octyl-beta-glucoside, Nonidet P-40 and CHAPS. A method, based on the preferential precipitation of 125I-RBP-receptor complex with poly(ethylene glycol) 8000, was developed in order to measure the RBP-binding activity in the detergent extracts. The receptor was fairly sta...
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