نتایج جستجو برای: 3t3

تعداد نتایج: 9508  

Journal: :Journal of virology 1975
P Jolicoeur D Baltimore

Titration of N- and B-tropic murine leukemia viruses on sensitive and resistant cell lines has been studied by direct XC plaque assay and infective center assay. The titration of cloned B-tropic virus by infective center assay on BALB/3T3 (Fv-1b/b) and NIH/3T3 (Fv-1n/n) cells gave one-hit patterns, with 100-fold less infected NIH/3T3 cells than BALB/3T3 cells. The titration of B-tropic virus on...

Journal: :Virology 1997
S Zhang V R Racaniello

Mouse cells are not susceptible to infection with echovirus 1 (EV-1) because they lack the viral receptor, human VLA-2. Two mouse fibroblast cell lines, L cells and 3T3 cells, were made susceptible to EV-1 infection after transformation with cDNAs of human VLA-2. After EV-1 infection, L cell transformants of human VLA-2 (alpha2beta1 L cells) develop cytopathic effect (CPE) as expected, while 3T...

ژورنال: :مجله دانشگاه علوم پزشکی بابل 0
علیرضا شمس، ar shams, آدرس: قزوین، بلوار شهید باهنر، دانشگاه علوم پزشکی قزوین، گروه آناتومی، تلفن: 3336001-0281، e-mail: [email protected] نسیم فرقانی، n forghani, فرشته آقائی، f aghaee, پریچهر یغمائی، p yagmaee عبدالرحمن رحیمیان ar rahimian

سابقه و هدف: کاربرد سلول های بنیادی در تحقیقات پزشکی و درمان افق جدیدی در بهبود بسیاری از بیماری ها ایجاد کرده است. لذا روش هایی که موجب جداسازی، کشت و نگاهداری بهتر این سلولها گردد، دارای اهمیت ویژه ای می باشد. هدف از این تحقیق بررسی اثر حمایتی سه لایه تغذیه کننده سلول های فیبروبلاستی جنینی (mef)، سلول های بنیادی مزانشیمی (msc) و رده سلولی 3t3 در جداسازی و کشت سلول های بنیادی جنینی جهت افزایش ...

Journal: :The Biochemical journal 1979
D R Bethell A E Pegg

1. The induction of ornithine decarboxylase activity in mouse 3T3 fibroblasts or an SV-40 transformed 3T3 cell line by serum was prevented by addition of the naturally occurring polyamines putrescine (butane-1,4-diamine) and spermidine. Much higher concentrations of these amines were required to fully suppress ornithine decarboxylase activity in the transformed SV-3T3 cells than in the 3T3 fibr...

Journal: :journal of sciences islamic republic of iran 0

total protein and dna extracted from histologically diagnosed normal nonmalignant and esophageal tumor tissues were used for analysis of polypeptides pattern by two-dimensional gel electrophoresis and dna transforming activity in nih 3t3 cell transfection assay, respectively. in comparison to normal tissues, eight polypeptides underwent down-regulation or disappeared, while seven polypeptides w...

Journal: :Investigative ophthalmology & visual science 2011
Naoko Okada Tetsuya Kawakita Kenji Mishima Ichiro Saito Hideyuki Miyashita Satoru Yoshida Shigeto Shimmura Kazuo Tsubota

PURPOSE Although the cornea expresses high levels of clusterin (CLU), the role of CLU in the cornea is poorly understood. This study was performed to investigate the possible role of CLU in corneal epithelial homeostasis. METHODS CLU was overexpressed in 3T3 cells by transfection of a vector encoding full-length CLU (Clu-3T3). Colony-forming efficacy (CFE) was compared in mouse corneal cell l...

Journal: :The Journal of Cell Biology 1976
H Gershman J Drumm L Culp

The sorting-out behavior (self-segregation of two cell types from mixtures of the two) of five different established cell lines was studied. Eight of the ten possible binary combinations of these lines, cultured as cellular aggregates, were examined. Mouse BALB/c 3T3 cells sorted out internally to the corresponding malignant SV40 virus-transformed 3T3 cells. The transformed 3T3 line (SVT-2) did...

2012
Yun-Ki Kim Jun-Ho Ahn Michael Lee

Recently, we reported that defective autophagy may contribute to the inhibition of the growth in response to PP2 (4-amino-5-(4-chlorophenyl)-7-(t-butyl)pyrazolo[3,4-d]pyrimidine), a selective SFK inhibitor, in multidrug-resistant v-Ha-ras-transformed NIH 3T3 cells (Ras-NIH 3T3/Mdr). In this study, we demonstrated that PP2 induces LC3 conversion via a mechanism that is uncoupled from autophagy a...

Journal: :Cancer research 1992
P G Johnston C M Rondinone D Voeller C J Allegra

The 3T3-L1 cell line is a preadipocyte cell line derived from the Swiss 3T3 mouse fibroblast cell line. We have compared the effect of 3T3-L1 conditioned medium (3T3-L1 CM) and Swiss 3T3 conditioned medium (3T3 CM) on the growth of normal mouse mammary cells (NMMG) and the human MCF-7 breast carcinoma cell line. 3T3 CM increased the growth of both NMMG and MCF-7 cells by 19 +/- 2% (SD) and 24 +...

Journal: :فصلنامه علمی پژوهشی گیاهان دارویی 0
f hadjiakhoondi department of pharmacognosy and medicinal plants research center, faculty of pharmacy, tehran university of medical sciences sn ostad department of toxicology and pharmacology, faculty of pharmacy, tehran university of medical sciences m khanavi department of pharmacognosy and medicinal plants research center, faculty of pharmacy, tehran university of medical sciences a hadjiakhoondi department of pharmacognosy and medicinal plants research center, faculty of pharmacy, tehran university of medical sciences, tehran, iran tel & fax: +98 - 21 - 66483626 b farahanikia department of pharmacognosy and medicinal plants research center, faculty of pharmacy, tehran university of medical sciences a salarytabar department of pharmacognosy and medicinal plants research center, faculty of pharmacy, tehran university of medical sciences

background: numerous molecules in papaveraceae family display interesting cytotoxic activities against tumor cell lines in vitro and hints of anticancer activities in vivo have been reported in a few cases. objective: numerous molecules in this family display interesting cytotoxic activities against tumor cell lines in vitro and hints of anticancer activities in vivo have been reported in a few...

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