The actin cytoskeleton is sensitive to changes in calcium, which affect contractility, actin-severing proteins, actin-crosslinking proteins and calmodulin-regulated enzymes. To dissect the role of calcium control on the activity of individual proteins from effects of calcium on other processes, calcium-insensitive forms of these proteins were prepared and introduced into living cells to replace...

Oleoyl-acyl carrier protein (18:1-ACP) thioesterase has been partially purified from developing safflower (Carthamus tinctorius) seeds. Protein species with molecular masses of 34 and 40 kD associated with thioesterase activity were identified and partially sequenced. Analysis of amino-terminal and internal cyanogen bromide peptide sequences revealed no differences in the primary structure of t...

The effect of membrane molecular weight cut off (MWCO) at three levels (10, 20 & 50 kD) on dynamic behavior of permeate flux (JP), hydraulic resistances (total hydraulic resistance, RT; reversible fouling resistance, Rrf; irreversible fouling resistance, Rif and membrane hydraulic resistance, Rm) and milk solutes rejection (protein, R<s...

the effect of membrane molecular weight cut off (mwco) at three levels (10, 20 &amp; 50 kd) on dynamic behavior of permeate flux (jp), hydraulic resistances (total hydraulic resistance, rt; reversible fouling resistance, rrf; irreversible fouling resistance, rif and membrane hydraulic resistance, rm) and milk solutes rejection (protein, rp; fat, rf; lactose, rl; minerals, rm and total solids, r...

1. Little information is currently available on protein turnover during chronic protein loss situations. We have thus measured the whole-body and tissue protein fractional synthesis rates (ks), the whole-body fractional protein degradation rate (kd), the capacity for protein synthesis (Cs) and the efficiency of protein synthesis (kRNA) in vivo in fed and fasted (1, 5 and about 9 days) 400 g rat...

A genomic clone (p268c) coding for the 28 kD storage protein Zc2 from maize endosperm has been isolated and sequenced. Formerly known as 28 kD glutelin-2 protein in previous works (1, 2), the sequence of the cDNA and of another genomic clone has been published. In this record, we report 1120 bp corresponding to the far upstream sequence reaching 1709 nucleotides from the first codon. The coding...

DNA replication in mammalian cells occurs in discrete nuclear foci. Here we show that terminally differentiated myotubes can be induced to reenter S phase and show the same pattern of replication foci as cycling cells. We used this cellular system to analyze the interaction of cell cycle proteins with these foci in vivo. Cyclin A and cdk2, but not cyclin B1 and cdc2, were specifically localized...

The two subtypes of melanomas, uveal (UM) and cutaneous (CM), originate from melanocyte transformation, but differ in their genetic etiology signaling pathways. MCL1 BCL2, the anti-apoptotic members BCL2 family, induce anti-cancer treatment resistance. Here, we evaluate basal levels role inducing response to inhibitor (MCL1i) CM UM. We used vitro assays (viability, immunoblot shRNA), bioinforma...

Abstract Background In 2017, it was recognized that there significant variation in the management and follow-up of Kawasaki disease (KD) at our institution, resulting inefficient use hospital resources patient confusion. A multidisciplinary team from cardiology, rheumatology, emergency medicine, infectious diseases (ID), general paediatrics created a standardized protocol distributed on October...