Bovine pancreatic deoxyribonuclease A was selectively cleaved by digestion with a-chymotrypsin under carefully controlled conditions. The peptide bond in DNase A between tryptophan residue 178 and serine 179 was quantitatively hydrolyzed in less than 15 min by 0.5 % ac-chymotrypsin at pH 8.5 and 4” when divalent metal ions were absent. The modified DNase A (DNase A (l-178,179-257)) was composed...

Abstract Introduction Peyronie’s Disease (PD) is characterized by a fibrous scar of the tunica albuginea. PD typically imaged with 2D ultrasound. We propose classification system these ultrasound findings in evaluation PD. Objective The purpose this study to establish functional sonographic for evaluating and examine relationship between grading curvature, duration PD, vascular erectile status....

Abstract Introduction The collaborative UK Emergency Laparotomy and Frailty (ELF) study was one of the first to investigate older adult population undergoing emergency surgery. Despite accounting for almost half laparotomies many considered high-risk, there remains a paucity research in this population. One undefined area is patient who presents with acute abdominal pathology treatable by lapar...

AIM To explore the role of complement system in the T cell activation. METHODS OVA(257-264) CTL epitope and C3b(AN42) encoding genes were synthesized and cloned into expression vector pEGFP-N1. COS-7 cells were transfected with the recombinant plasmid pEGFP-N1. The expression of the fusion protein was determined by FACS. Twenty-four hours after transfection, cultural supernatants of the trans...

PURPOSE To evaluate the safety of outpatient endovascular treatment in patients with multiple sclerosis (MS) and chronic cerebrospinal venous insufficiency (CCSVI). MATERIALS AND METHODS A retrospective analysis was performed to assess complications occurring within 30 days of endovascular treatment of CCSVI. The study population comprised 240 patients; 257 procedures were performed over 8 mo...

To study the requirements for assembly of MHC class I molecules with antigenic peptides in the endoplasmic reticulum (ER), we studied Ag processing in insect cells. Insects lack a class I recognition system, and their cells therefore provide a "blank slate" for identifying the proteins that have evolved to facilitate assembly of class I molecules in vertebrate cells. H-2Kb heavy chain, mouse be...

ENWEndNote BIBJabRef, Mendeley RISPapers, Reference Manager, RefWorks, Zotero AMA Stefan M, Zapolnik P, Pyrkosz A. Deletion of the RNF6 gene in a patient with epileptic encephalopathy – case report and literature review. Pediatria Polska - Polish Journal Paediatrics. 2022:257-261. doi:10.5114/polp.2022.120119. APA Stefan, M., Zapolnik, P., & Pyrkosz, (2022). Paediatrics, 257-261. https://doi.or...