Cell-suspension cultures of Capsicum annuum L., Solanum aculeatissimum Jacq. and, Datura fastuosa L. (Solanaceae) were established and investigated for the ability to biotransform exogenously-supplied hydroquinone to arbutin. All cultures exhibited good 24-day growth in Murashige and Skoog medium, supplemented with 2,4-dichlorophenoxyacetic acid and benzylaminopurine as plant growth regulators,...

A gene (EGLI) encoding an endo-p-l,4-~-glucanase (EGase, EC 3.2.1.4) of pea (Pisum sativum) has been cloned and characterized. EGL 1 encodes a 486-amino acid polypeptide, including a 24-mer putative signal peptide. The mature protein has a calculated molecular mas of 51.3 kD and an isoelectric point of 9.1. This pea ECase shares significant similarity with ECases from other plant species, but i...

In vitro translation products of polyadenylated RNA from untreated and auxin-treated basal sections of soybean (Glycine max var. Wayne) hypocotyl were analyzed by two-dimensional polyacrylamide gel electrophoresis. Within one hour of 2,4-dichlorophenoxyacetic acid treatment, the translatable messenger RNAs for at least twelve in vitro translation products are modulated upward. In vitro translat...

Using recently developed techniques for solubilization of RNA polymerase from soybean chromatin and isolation of plasma membrane fractions from plants we can show the presence of a transcriptional factor specifically released from the membranes by auxin, 2,4-dichlorophenoxyacetic acid. The nonauxin, 3,5-dichlorophenoxyacetic acid, does not release the factor, but subsequent exposure of the memb...

This Note questions the safety of crop plants engineered with transgenes coding for the degradation of the herbicide 2,4-dichlorophenoxyacetic acid (2,4-D) into its cytotoxic metabolite 2,4-dichlorophenol (2,4-DCP).

Wheat husks (Fagopyrum esculentum) were modified by treatment with sulfuric acid. The precursor material (FEWS) and the (TFEWS) characterized different techniques to identify structural changes promoted chemical treatment. Subsequently, TFEWS was applied as an adsorbent remove 2,4-dichlorophenoxyacetic acid (2,4-D) pesticide from aqueous solutions. Adsorption studies considered pH effects dosag...