One challenge in studying the second messenger inositol(1,4,5)-trisphosphate (InsP₃) is that it is present in very low amounts and increases only transiently in response to stimuli. To identify events downstream of InsP₃, we generated transgenic plants constitutively expressing the high specific activity, human phosphatidylinositol 4-phosphate 5-kinase Iα (HsPIPKIα). PIP5K is the enzyme that sy...

sclerotinia sclerotiorum is a phytopathogenicfungus that attacks more than 400 plant species of them medicinal plant. in this study the extract obtained from basal stalk rot of helianthus annuus l., attacked by s. sclerotiorum, was subjected to the analysis of ftir to identify the presence of beta-glucan. ft-ir spectrum showed four ranges of bands in 890 cm-1, two overlap band in 1047 and 1078 ...

Phagocytes engulf pathogenic microbes, kill them and degrade their cellular macromolecules by hydrolytic enzymes in phagolysosomes. However, such enzymes are unable to degrade some microbial polysaccharides, and fate of such indigestible polysaccharides in phagocytes remains uncertain. Using the extracellular domain of Dectin-1 as β-glucan-specific probes, we succeeded in detection of soluble a...

Background: Findings about the effects of -glucan on serum lipoproteins are conflicting. Objective: The study investigated the effects of -glucan from oat bran in bread and cookies (study 1) and in orange juice (study 2) on serum lipoproteins in mildly hypercholesterolemic subjects. Design: In study 1, 48 subjects (21 men, 27 women) received for 3 wk control bread and cookies rich in wheat fibe...

Three experiments were conducted to investigate the effects of dietary supplementation of a ß-(1,3)(1,6)-D-glucan derived from the yeast Saccharomyces cerevisiae, on growth performance parameters and intestinal functionality in mirror carp (Cyprinus carpio L.) and on progression of a chronic, pre-existing mycobacteriosis in zebrafish (Danio rerio). The 1 experiment revealed that mirror carp fed...

The apoptotic effect of bacteria-derived beta-glucan was investigated in human colon cancer cells SNU-C4 using terminal deoxynucleotidyl transferase (TdT)-mediated dUTP nick end labeling (TUNEL) assay, reverse transcription-polymerase chain reaction (RT-PCR) expressions of Bcl-2, Bax, and Caspase-3 genes, and assay of caspase-3 enzyme activity. beta-Glucan of 10, 50, and 100 microg/mL decreased...

This study aimed to evaluate the effects of β-glucan ingestion (Saccharomyces cerevisiae) on the plasmatic levels of tumor necrosis factor-α (TNF-α) and interleukin-10 (IL-10), alveolar bone loss, and pancreatic β-cell function (HOMA-BF) in diabetic rats with periodontal disease (PD). Besides, intestinal morphology was determined by the villus/crypt ratio. A total of 48 Wistar rats weighing 203...

PURPOSE In vitro beta-glucan can enhance tumor cytotoxicity through iC3b receptors on leukocytes. We tested if (1-->3),(1-->4)-beta-D-glucan (beta-glucan) can synergize with anti-GD2 monoclonal antibody (MoAb) 3F8 (mouse IgG3) in therapy of human neuroblastoma xenografts. EXPERIMENTAL DESIGN Athymic nude mice with established neuroblastoma xenografts were treated with daily i.p. or p.o. beta-...

Introduction: The increasing consumption of high-fat and high-fructose foods contributes to the prevalence global obesity. Low-grade chronic inflammation in obesity is a significant risk factor for insulin resistance type 2 diabetes. Therefore, this study aimed determine effect β-(1,3)-D-glucan from oyster mushroom (Pleurotus ostreatus) extract on rats fed with diet. Design Methods: This experi...

β-Glucan is a dietary fibre, found in many natural sources, and controls chronic metabolic diseases effectively. However, β-glucan from the yeast has rarely been investigated. Objectively, conditions were optimized to isolate β-glucan from the yeast (max. 66% yield); those optimized conditions included 1.0 M NaOH, pH 7.0, and 90°C. The purity and identity of the isolated β-glucan were character...