We examined whether ATP stimulation of P2X purinoceptors would raise blood pressure in decerebrate cats. Femoral arterial injection of the P2X receptor agonist alpha,beta-methylene ATP into the blood supply of the triceps surae muscle induced a dose-dependent increase in arterial blood pressure. The maximal increase in mean arterial pressure (MAP) evoked by 0.1, 0.2, and 0.5 mM alpha,beta-methy...

The increased unidirectional sodium influx found when human erythrocytes are suspended in isotonic salt solutions containing bicarbonate ions as a replacement for chloride ions was examined. The increased sodium movement appears to have the transport characteristics of anion movement. Inhibitors of anion transport such as furosemide, fluorodinitrobenzene (FDNB), and 4-acetamido-4'-isothiocyano-...

We studied the regulation of intracellular pH (pH i ) in single cultured astrocytes passaged once from the hippocampus of the rat, using the dye 2 9 ,7 9 -biscarboxyethyl-5,6-carboxyfluorescein (BCECF) to monitor pH i . Intrinsic buffering power ( b I ) was 10.5 mM (pH unit) 2 1 at pH i 7.0, and decreased linearly with pH i ; the best-fit line to the data had a slope of 2 10.0 mM (pH unit) 2 2 ...

A novel stilbene disulfonate, 4-trimethylammonium-4'-isothiocyanostilbene-2,2'-disulfonic acid (TIDS), has been chemically synthesized, and the interaction of this probe with human erythrocyte anion exchanger (AE1) was characterized. Covalent labeling of intact erythrocytes by [N(+)((14)CH(3))(3)]TIDS revealed that specific modification of AE1 was achieved only after removal of other ligand bin...

In order to influence the fermentation pattern of Propionibacterium freudenreichii towards enhanced propionate formation, growth and product formation with glucose and lactate as energy sources were studied in a three-electrode poised-potential amperometric culture system. With anthraquinone 2,6-disulfonic acid (E(0)' = -184 mV; poised electron potential = -224 mV) or cobalt sepulchrate (E(0)' ...

Main-chain polymers of chiral imidazolidinone were successfully synthesized by reaction of chiral imidazolidinone dimers with disulfonic acid. Chiral imidazolidinones were incorporated into the main-chain of the polymer by ionic bonding. These polymers could be used as polymeric chiral organocatalysts for asymmetric Diels-Alder reactions.

We use cyclic charge-discharge experiments to evaluate the capacity retention rates of two quinone-bromide flow batteries (QBFBs). These aqueous QBFBs use a negative electrolyte containing either anthraquinone-2,7-disulfonic acid (AQDS) or anthraquinone-2-sulfonic acid (AQS) dissolved in sulfuric acid, and a positive electrolyte containing bromine and hydrobromic acid. We find that the AQS cell...

The addition of 1,4-diazabicyclo-[2,2,2]octane (DABCO) (100 mM) or 1,2-dihydroxybenzene-3,5-disulfonic acid (Tiron) (1 mM) to a reaction mixture containing 10 mM linoleic acid (LA), 20% EtOH, and 135 microM dehydroascorbic acid (DHA) as a catalyst suppressed LA peroxidation, but the addition of mannitol (approximately 100 mM), uric acid (100 microM), and catalase (6.5 units) did not. DHA or 2,3...