background: embryo cryopreservation is the process that water is removed from the cell by cryoprotectant materials, and embryos are stored at temperature below zero. this process may affect the viability and developmental potential of embryos. objective: in this study, the effect of the vitrification cryotop method on the expression level of oct4 and mest developmental genes in mouse blastocyst...

abstract background: research studies on reproductive mechanism of laboratory animals are essential for further advancement of assisted reproductive techniques (art). one of these studies includes the assessment of in-vitro development of pre-implantation embryos. the objective was to compare the cleavage rates and morphology of in-vivo formed 2 to 8 cell embryos and blastocysts with in-vitro c...

background: timing of the first zygotic cleavage is an accurate predictor of embryo quality. embryos that cleaved early (ec) have been shown to exhibit higher developmental viability compared to those that cleaved at a later period (lc). however, the viability of ec embryos in comparison to lc embryos after vitrification is unknown. the present study aims to investigate the post-vitrification d...

Background This study was designed to develop a new co-culture system, assess the effect of luteinizing hormone (LH) using sequential media to promote development and increase the quality of 2-cell murine embryos through the 8-16 cell stage to morula and blastocyst stages. MaterialsAndMethods Monolayers for co-culture were prepared from ovine oviduct epithelial cells (OOEC) in DMEM/F12 medium a...

objective: the aim of the study was to compare the effects of two different concentrations of cryoprotectants by cryotopvitrification on survival, developmental capacity and heat shock protein 72 (hsp72) expression of two-cell mouse embryos. materials and methods: in this experimental study, transcript analysis of hsp72 gene was performed on non-vitrified and vitrified 2-cell mouse embryos via ...

the study was undertaken to elucidatethe beneficial effects of goat follicular fluid (gff) added to maturation and culture media on in vitro maturation, fertilization and post-fertilization development of oocytes in black bengal goats. follicular fluid and oocytes were collected from slaughter house goat’s ovaries using the aspiration technique. media were prepared using tcm-199 supplemented wi...

background: using cellular phone has rapidly increased all over the world. also, the concern on the possible health hazards of electromagnetic fields (emf) induced from cell phones to reproduction has been growing in many countries. the aim of this study was to assess the consequences and effects of exposure to the cell phone radiation on the quality and survival rates of preimplantation embryo...

introduction: the value of embryonic stem-like cells in cloning is obvious. production of cloned animals can be achieved by introduction of these cells into a tetraploid embryo. tetraploid embryo is used as a feeder for development of embryonic stem-like cells and can be produced in vitro by electro-fusion of 2-cell embryos. the aim of this study was to ass,essthe effect of voltage alteration a...