This article is part of a Minifocus on Ubiquitin. For further reading, please see related articles: ʻUbiquitin and SUMO in DNA repair at a glanceʼ by Helle D. Ulrich (J. Cell Sci. 125, 249-254), ʻEmerging regulatory mechanisms in ubiquitin-dependent cell cycle controlʼ by Annamaria Mocciaro and Michael Rape (J. Cell Sci. 125, 255-263), ʻThe role of ubiquitylation in receptor endocytosis and end...

Very high concentrations of CA 125 have been found in some ovarian cancer patients after repeated radioimmunodetection with anti-CA 125 antibodies [OC125-F(ab')2]. In one patient we measured a CA 125 concentration of 135,000 kilo-arb. units/L, using an enzyme immunoassay involving OC125 antibodies. With an immunoradiometric assay involving use of two new anti-CA 125 antibodies (B43.13 and B27.1...

HRP has been used as a cytochemical marker for a sterelogic analysis of pinocytic vesicles and secondary lysosomes in cultivated macrophages and L cells. Evidence is presented that the diaminobenzidine technique (a) detects all vaculoes containing encyme and (b) distinguishes between incoming pinocytic vesicles and those which have fused with pre-existing lysosomes to form secondary lososomes. ...

Atrazine (ATR), one of the most widely used herbicides worldwide, has caused a series of toxicological and environmental problems. This study sought to investigate the effects of ATR on the immune system of mice. Four-week-old female C57B l/6 mice were treated with 5, 25, and 125 mg/kg ATR for 28 days. On day 29, blood was collected and the spleen was harvested to detect lymphocyte transformati...

Phosphatase and tensin homologue deleted on chromosome 10 (PTEN) and Akt are important regulators of the phosphatidylinositol 3-kinase (PI3K) pathway and thus are important to the regulation of a wide spectrum of tumor-related biological processes. Akt regulates several critical cellular functions, including cell cycle progression; cell migration, invasion, and survival; and angiogenesis. Decre...

The aim of this study was to perform various tests of the anesthetic efficacy of eugenol applied to different handling tasks such as artificial insemination, spawning induction, obtaining body weight and length, the conduct of operations, biopsies and transport of Xiphophorus helleri (Heckel, 1848). The tests were performed on 10 individual males and 10 individual females of X. helleri -1 (swor...

OBJECTIVE To evaluate the role of lysophosphatidic acid (LPA) as a tumor marker in diagnosis and follow-up of patients with epithelial ovarian cancer. METHODS Eighty-seven epithelial ovarian cancer patients, 74 benign ovarian tumor patients, and 50 healthy women were enrolled in the study. Twenty-nine of 87 epithelial ovarian cancer patients were followed up for 6 cycles of paclitaxel-carbopl...

Background Lymphopenia is a marker of immunosuppression after severe coronavirus disease-2019 (COVID-19) which characterized by acute respiratory distress syndrome (ARDS). This study aimed to evaluate the relationships between persistent lymphopenia and ARDS. Methods A retrospective cohort 125 patients with COVID-19 admitted government-designated treatment center 14 January 2020, 20 March 2020 ...

Isotopic techniques were used to test the hypothesis that exercise and nitric oxide synthase (NOS) inhibition have distinct effects on tissue-specific fatty acid and glucose uptakes in a conscious, chronically catheterized mouse model. Uptakes were measured using the radioactive tracers (125)I-labeled beta-methyl-p-iodophenylpentadecanoic acid (BMIPP) and deoxy-[2-(3)H]glucose (DG) during tread...