Efficacy of inoculative freezing by ice nuclei in a simulated winter environment was studied in the wood frog (Rana sylvatica), a freeze-tolerant species that overwinters on the forest floor beneath organic detritus. Adult frogs were confined to plastic canisters and cooled to -2 degrees C over 24 hr with their ventral skin in contact with substrate (humic soil hydrated to 40, 10, or 5%, or soi...

BACKGROUND We recently showed that freeze-dried cells stored for 3 years at room temperature can direct embryonic development following cloning. However, viability, as evaluated by membrane integrity of the cells after freeze-drying, was very low; and it was mainly the DNA integrity that was preserved. In the present study, we improved the cells' viability and functionality after freeze-drying....

Natural and artificial materials including rocks and cement-based materials such as concrete and cement mortar are affected both physically and chemically by various natural factors known as weathering factors. The freeze-thaw process, as a weathering factor, considerably affects the properties of rocks and concrete. Therefore, the effect of the freeze-thaw process on the physical and mechanica...

Membrane splitting by freeze-fracture (2) could provide chemical information about the transmembrane distribution of constituents if the individual halves of the membrane could be analyzed separately. One means of analysis is the detection of membraneincorpora ted radioisotopic labels by autoradiography after freeze-fracturing (6). We have examined three model systems, two nonbiological and one...

The present study has found that dried potato samples yielded significantly higher levels of steroidal alkaloids such as α-solanine and α-chaconine than the corresponding fresh samples, as determined by the UPLC-MS/MS technique. Among the drying techniques used, air drying had the highest effect on steroidal alkaloid contents, followed by freeze drying and vacuum oven drying. There was no signi...

Orphanides A., Goulas V., Gekas V. (2013): Effect of drying method on the phenolic content and antioxidant capacity of spearmint. Czech J. Food Sci., 31: 509–513. The changes in total phenolics, hydroxycinammic acid derivatives, and antioxidant properties of spearmint after five drying treatments (convection oven drying, freeze-drying, microwave drying, and air drying with the sun exposure and ...

Six direct plating media were evaluated for their suitability to recover uninjured, heat-injured, and freeze-injured cells of four strains of Listeria monocytogenes from four foods. Cells were inoculated into foods to achieve ca. 10(2) to 10(3), 10(4) to 10(5), or 10(5) to 10(6) viable cells per ml or g (low, medium, and high populations, respectively). No appreciable differences in recovery of...

Waste tires, rubbers, plastic and steel materials, normally produced in every society, enter the environment and cause serious problems. These problems may, to some extent, be reduced by finding applications for them in engineering, for example, they can be used for geotechnical applications as backfill material and solving problems with low shear strength soils. Such materials may be subjected...

In this study, the content of total polyphenols (TP) and antioxidant activity (AA) fresh herbs (13 in total) those subjected to freeze-drying process (stems leaves separately) were compared. Moreover, TP AA retail, commercial food spices (19 two leading companies on Polish market The novelty our studies is comparison between dried forms additionally a commercially available (in forms). It was f...

The purpose of this study was to obtain the best anthocyanin pigment extract powder from 3 types of drying process, vacuum drying, spray drying, and  freeze-drying. The duration and temperature for each type of drying process are 15 h with temperature of 40 oC, 2 h with temperature of 120 oC (inlet) and 60 oC (outlet), 24 h with temperature of -50 o</su...