WRKY transcription factors are known to function in a number of plant processes. Here we have characterized 15 WRKY family genes of the important ornamental species chrysanthemum (Chrysanthemum morifolium). A total of 15 distinct sequences were isolated; initially internal fragments were amplified based on transcriptomic sequence, and then the full length cDNAs were obtained using RACE (rapid a...

The short-day plants Begonia cheimantha, Chrysanthemum morifolium (one pot and one cut cultivar), Euphorbia pulcherrima (poinsettia) and Kalanchoe blossfeldiana were grown at vapour pressure de®cits (VPDs) of 155, 420 and 660 Pa (corresponding to 93, 81 and 70% relative air humidity, respectively). The lowest VPD delayed time to ̄owering by three to four days in pot and cut chrysanthemum but di...

Chrysanthemum stunt viroid (CSVd) was inoculated into two chrysanthemum (Chrysanthemum morifolium) cultivars, the CSVd-susceptible cultivar Piato and the CSVd-resistant cultivar Mari Kazaguruma. For CSVd inoculation, grafting and Agrobacterium-mediated inoculation were used. In grafting experiments, CSVd was detectable in Mari Kazaguruma after grafting onto infected Piato, but after removal of ...

Chrysanthemums (Chrysanthemum morifolium) are susceptible to tobacco mosaic virus (TMV). TMV-based expression vectors have been used in high-throughput experiments for production of foreign protein in plants and also expressing green fluorescent protein (GFP) to allow visualization of TMV movement. Here, we used TMV expressing the GFP to examine the infection of chrysanthemum by a TMV-based exp...

The white petals of chrysanthemum (Chrysanthemum morifolium Ramat.) are believed to contain a factor that inhibits the accumulation of carotenoids. To find this factor, we performed polymerase chain reaction-Select subtraction screening and obtained a clone expressed differentially in white and yellow petals. The deduced amino acid sequence of the protein (designated CmCCD4a) encoded by the clo...

Chrysanthemum has been cultivated for more than 2000 years and today, it is the world’s second most economically important floricultural crop following the rose (Teixeira da silva, 2003). Due to high popularity and demand for chrysanthemum it has become one of the first commercial targets for Micro propagation and thus tissue culture can be utilized for its large scale production. Regeneration ...

This research used auto/biographical interviews to explore the experiences of 19, 8 to 31 year olds who had a parent with dementia. Thematic analysis revealed challenges occasioned by the master narrative that people with dementia are 'still' the same person they were prior to the onset of their condition. While this notion is - rightly - at the heart of person-centered care in dementia service...