Both protein kinase C (PKC) and adenosine receptor activation have been shown to enhance ATP-sensitive K+(KATP) channels. The present studies were designed to determine whether PKC mediates adenosine effects on the KATP channel. The dependence of KATP channel activity ( nP o) on intracellular ATP concentration ([ATP]i) was determined in excised rabbit ventricular membrane patches. External aden...

ATP-sensitive K (KATP) channels are nucleotide-gated bioenergy sensors that enable high-fidelity feedback communication between cellular energy dynamics and membrane electric activity.1–4 Particularly dense in cardiac sarcolemma where they are composed through heteromultimeric assembly of inwardly rectifying K channel pores, typically KCNJ11-encoded Kir6.2 proteins, and regulatory ATPbinding ca...

Vascular ATP-sensitive potassium (KATP) channels have an important role in hypoxic vasodilation. Because KATP channel activity depends on intracellular nucleotide concentration, one hypothesis is that hypoxia activates channels by reducing cellular ATP production. However, this has not been rigorously tested. In this study we measured KATP current in response to hypoxia and modulators of cellul...

Medial temporal lobe structures are essential for memory formation which is associated with coherent network oscillations. During ontogenesis, these highly organized patterns develop from distinct, less synchronized forms of network activity. This maturation process goes along with marked changes in intrinsic firing patterns of individual neurons. One critical factor determining neuronal excita...

BACKGROUND Pancreatic beta cells express ATP-sensitive potassium (KATP) channels that are needed for normal insulin secretion and are targets for drugs that modulate insulin secretion. The KATP channel is composed of two subunits: a sulfonylurea receptor (SUR 1) and an inward rectifying potassium channel (Kir6.2). KATP channel activity is influenced by the metabolic state of the cell and initia...

Intense research is conducted to identify new molecular mechanisms of angiogenesis. Previous studies have shown that the angiogenic effects of hydrogen sulfide (H2S) depend on the activation of ATP-sensitive potassium channels (KATP) and that C-type natriuretic peptide (CNP), which can act through KATP, promotes endothelial cell growth. We therefore investigated whether direct KATP activation i...

Congenital hyperinsulinism is a condition of dysregulated insulin secretion often caused by inactivating mutations of the ATP-sensitive K+ (KATP) channel in the pancreatic beta cell. Though most disease-causing mutations of the 2 genes encoding KATP subunits, ABCC8 (SUR1) and KCNJ11 (Kir6.2), are recessively inherited, some cases of dominantly inherited inactivating mutations have been reported...

Understanding the etiology of and development of appropriate treatment for myocardial ischemia and associated sudden cardiac death is a major goal of cardiovascular research. In a recent review,1 a compelling case is presented that sarcolemmal ATP-sensitive potassium (KATP) channels are an excellent target for antiarrhythmic therapy. Because opening of cardiac KATP channels shortens the action ...

26 The pancreatic ATP-sensitive potassium (KATP) channel consisting of four Kir6.2 and 27 four sulfonylurea receptor SUR1 subunits play a key role in insulin secretion by 28 linking glucose metabolism to membrane excitability. Syntaxin 1A is a plasma 29 membrane protein important for membrane fusion during exocytosis of insulin 30 granules. Here, we show that syntaxin 1A and KATP channels endog...

SINCE THEIR DISCOVERY OVER 25 years ago, ATP-sensitive K (KATP) channels have been studied extensively in many tissues, including heart, pancreas, brain, skeletal and smooth muscle, pituitary, and kidney (6, 10, 18, 19). KATP channels are critical sensors that couple cellular metabolic state to membrane excitability and organ function and regulate a wide range of cellular functions including ho...