DNA helicases unwind or rearrange duplex DNA during replication, recombination and repair. Helicases of many pathogenic organisms such as viruses, bacteria, and protozoa have been studied as potential therapeutic targets to treat infectious diseases, and human DNA helicases as potential targets for anti-cancer therapy. DNA replication machineries perform essential tasks duplicating genome in ev...

In eukaryotes, two Mcm2–7 rings are loaded onto DNA at origins of replication during G1 phase1,2. The double hexamer is inactive as a heli­ case but is activated upon entry into S phase by the Dbf4­dependent Cdc7 kinase, cyclin­dependent kinase and several transient factors that activate origins; subsequently, Cdc45 and GINS assemble onto each Mcm2–7 complex, thereby forming two active CMG heli...

Replicative helicases in all cell types are hexameric rings that unwind DNA by steric exclusion in which the helicase encircles the tracking strand only and excludes the other strand from the ring. This mode of translocation allows helicases to bypass blocks on the strand that is excluded from the central channel. Unlike other replicative helicases, eukaryotic CMG helicase partially encircles d...

BACKGROUND Between 5% and 10% of patients die or are urgently readmitted within 30 days of discharge from hospital. Readmission risk indexes have either excluded acute diagnoses or modelled them as multiple distinct variables. In this study, we derived and validated a score summarizing the influence of acute hospital diagnoses and procedures on death or urgent readmission within 30 days. METH...

The purpose of this paper is to examine whether the presence of singular CMG configurations poses an obstruction to the small-time local controllability (STLC) and stabilizability of the attitude dynamics of a spacecraft actuated by single-gimbal CMGs. We identify a class of singular CMG configurations called critically singular configurations, at which the linearized dynamics fail to be contro...

Replicative helicases are ring-shaped hexamers that encircle DNA for duplex unwinding. The currently accepted view of hexameric helicase function is by steric exclusion, where the helicase encircles one DNA strand and excludes the other, acting as a wedge with an external DNA unwinding point during translocation. Accordingly, strand-specific blocks only affect these helicases when placed on the...

The CMG complex composed of Mcm2-7, Cdc45 and GINS is postulated to be the eukaryotic replicative DNA helicase, whose activation requires sequential recruitment of replication proteins onto Mcm2-7. Current models suggest that Mcm10 is involved in assembly of the CMG complex, and in tethering of DNA polymerase α at replication forks. Here, we report that Mcm10 is required for origin DNA unwindin...

Graphene-based sheets show promise for a variety of potential applications, and researchers in many scientific disciplines are interested in these materials. Although researchers have developed many ways of generating single atomic layer carbon sheets, chemical exfoliation of graphite powders to graphene oxide (GO) sheets followed by deoxygenation to form chemically modified graphene (CMG) offe...

We identified primary cilia and centrosomes in cultured human umbilical vein endothelial cells (HUVEC) by antibodies to acetyl-alpha-tubulin and capillary morphogenesis gene-1 product (CMG-1), a human homologue of the intraflagellar transport (IFT) protein IFT-71 in Chlamydomonas. CMG-1 was present in particles along primary cilia of HUVEC at interphase and around the oldest basal body/centriol...

In this article, we review our efforts to continuously tune mechanical and thermal properties in multilayer chemically modified graphene (CMG) films. An alteration of the graphene lattice by functional groups, by defects created during reduction, or by defect re-crystallization is used to control CMG mechanical and thermal properties. We attribute a notable increase in Young’s modulus and film ...