The occurrence and removal of 49 antibiotics 11 selected antibiotic resistance genes (ARGs) were investigated in 2 vertical subsurface flow (VF) constructed wetlands (1.5 m2 each): an unsaturated (UVF) unit a partially saturated (SVF) (0.35 m out 0.8 m) operating parallel treating urban wastewater. Thirteen detected influent wastewater, 6 which present all samples. SVF showed statistical signif...

strains were negative for qnrA, qnrB and qnrS and other studies have shown that qnr in A. baumannii is not widespread. 4 Four of the eight qnr genes were found in ciprofloxacin-resistant isolates and three in isolates with intermediate susceptibility. C. freundii harboured qnrB8 and was ciprofloxacin-susceptible. Six of the eight (75%) qnr-positive isolates were ESBL-producing strains represent...

Bovine mastitis is often taken as one of the most common diseases in dairy farms, which its pathophysiology leads to a reduction milk production and quality. The penetration pathogenic bacteria into mammary gland, through either contagious or environmental approach, has been determined way infection. derived have become challenge practice, since increasing exposure antimicrobial. In order ident...

In the sequence upstream from qnrB (but not qnrA or qnrS) is a LexA binding site. qnrB was shown to be under SOS control by demonstrating that quinolone susceptibility decreased with increasing temperature in a strain with a recA441(Ts) allele, whereas qnrB expression increased in response to ciprofloxacin or mitomycin C in strains with an intact lexA gene.

Real-time PCR assays were developed for the enumeration of plasmid-mediated quinolone resistance (PMQR) determinants, such as the qnrA, qnrB, and qnrS genes, in different water samples and chicken feces. The results indicate that the developed assays are specific and sensitive for the quantification of qnr genes in complex samples.

The accuracy of the MicroScan WalkAway, BD Phoenix, and Vitek-2 systems for susceptibility testing of quinolones and aminoglycosides against 68 enterobacteria containing qnrB, qnrS, and/or aac(6 ')-Ib-cr was evaluated using reference microdilution. Overall, one very major error (0.09%), 6 major errors (0.52%), and 45 minor errors (3.89%) were noted.

The presence of plasmid-mediated quinolone resistance genes [i.e., qnrA, qnrB, qnrS, aac(6')-Ib-cr, and qepA] was evaluated among 42 bla(KPC)-containing Klebsiella pneumoniae isolates collected in the eastern United States. One isolate carried the bla(KPC-3) and qnrB19 genes on the same conjugative plasmid, whereas another carried the bla(KPC-3) and qnrA1 genes on separate plasmids.

BACKGROUND Plasmid-mediated quinolone resistance genes (PMQR) have been shown to play not only an important role in quinolone resistance, but also resistance to other antibiotics, particularly β-lactams and aminoglycosides. These genes are mainly associated with clinical isolates of Enterobacteriaceae. However, detection of PMQR genes in the community isolates can increase the dissemination rat...

OBJECTIVES We describe 12 VIM-1-producing strains (7 Enterobacter cloacae, 2 Klebsiella pneumoniae and 3 clonal Klebsiella oxytoca strains) detected among clinically relevant Enterobacteriaceae isolates from routine cultures at the Hospital del Mar (Barcelona, Spain) from December 2006 to May 2007. METHODS Susceptibility to carbapenems was evaluated with the MicroScan system. beta-Lactamases ...