Compatible osmolytes are potent osmoprotectants that play a role in counteracting the effects of osmotic stress. Proline (Pro) is one of the most common compatible osmolytes in water-stressed plants. The accumulation of Pro in dehydrated plants is caused both by activation of the biosynthesis of Pro and by inactivation of the degradation of Pro. In plants, L-Pro is synthesized from L-glutamic a...

Osmotic stress-induced accumulation of proline, an important protective osmolyte in higher plants, is dependent on the expression of 1-pyrroline-5-carboxylate synthase (P5CS) and proline dehydrogenase (PDH) enzymes that catalyze the rate-limiting steps of proline biosynthesis and degradation, respectively. Proline metabolism is modulated by differential regulation of organ specific expression o...

The sosl mutant of Arabidopsis thaliana i s more than 20 times more sensitive to NaCl stress than wild-type Arabidopsis. Because proline (Pro) i s generally thought to have an important role in plant salt tolerance, the sosl mutant and the wild type were compared with respect to their capacity to accumulate Pro under NaCl stress, and sosl mutant plants accumulated more Pro than the wild type. T...

Chinese hamster ovary (CHO) cells are the leading mammalian cell host employed to produce complex secreted recombinant biotherapeutics such as monoclonal antibodies (mAbs). Metabolic selection marker technologies (e.g. glutamine synthetase (GS) or dihydrofolate reductase (DHFR)) routinely generate lines. Here we describe development of a system based on metabolic requirement CHO proline, and th...

abstract the purpose of this research was to transform two economically important cultivars of strawberry with p5cs gene, which encodes δ1-pyrroline-5-carboxylate synthetase (p5cs), the key enzyme in proline biosynthesis. shoots were obtained on ms basal medium supplemented with 2% glucose and 4 mg/l tdz for camarosa and kurdistan cultivars. for genetic transformation, a binary vector pbi121 co...

Salinity is a major limiting factor for plant growth and development. To evaluate the impact of P5CS gene expression under in vitro salt stress condition, transgenic tobacco (Nicotiana plumbaginifolia) carrying P5CS gene and non-transgenic plants were treated with 0, 100, 150, 200 or 250 mM NaCl for 28 days. Proline content, lipid peroxidation and the activity of some antioxidant enzymes after ...

This study was carried out to investigation the possibility of using Triadimefon (TRD) in order to decrease the adverse effects of salt stress on Medicago plant. Triadimefon is a member of Triazol compounds which enhances stress tolerance through physiological processes. Two cultivars of Medicago sitiva including Hamedani and Yazdi were used in this study. Plants were treated with 1, 2 and 4 mg...