NEMO is the regulatory subunit of the IkappaB kinase (IKK) in NF-kappaB activation, and its CC2-LZ region interacts with Lys63 (K63)-linked polyubiquitin to recruit IKK to receptor signaling complexes. In vitro, CC2-LZ also interacts with tandem diubiquitin. Here we report the crystal structure of CC2-LZ with two dimeric coiled coils representing CC2 and LZ, respectively. Surprisingly, mutagene...

Speaker recognition suffers severe performance degradation under noisy environments. To solve this problem, we propose a novel method based on morphological component analysis. This method employs a universal background dictionary (UBD) to model common variability of all speakers, a speech dictionary of each speaker to model special variability of this speaker and a noise dictionary to model va...

La planificación de unidades didácticas mediante el enfoque la UbD (Understanding by design), desarrollada por Wiggins y McTighe (2005), está teniendo una incursión lenta gradual en docencia, a pesar que esta obra es bastante citada diferentes trabajos académicos. Existe gran brecha entre lo se presenta teóricamente practica ejercicio docencia al respecto este enfoque. El presente trabajo ha pr...

A robust chemical ubiquitination method was developed. The method employed a genetically incorporated azidonorleucine as an orthogonal lysine precursor for the installation of a Gly residue bearing an Nα-auxiliary which mediated the ligation between ubiquitin(1-75)-thioester and the target protein. To demonstrate our methodology, a model protein, K48-linked diubiquitin, was synthesized with an ...

Abstract Wellbore instability causes main concerns in the oil and gas industry due to personnel safety overall expenditure. Several signs such as wellbore spalling, rate of penetration reduction, pipes sticking, well unnatural wash out, collapsing, induce fracture could indicate during drilling. A balance between stress concentration near rock strength is primary condition for a stability Thus,...

Interstitial cystitis/bladder pain syndrome (IC/BPS) is a debilitating urological condition that is resistant to treatment and poorly understood. To determine novel molecular treatment targets and to elucidate the contribution of the nervous system to IC/BPS, many rodent bladder pain models have been developed. In this study we evaluated the effects of anesthesia induction and temperature varia...

Left-sided colon carcinoma (LSCC) and right-sided colon carcinoma (RSCC) differ in their genetic susceptibilities to neoplastic transformation. The present study identified 11 genes that were differentially expressed in LSCC and RSCC by expression profiling with microarray analysis. Compared with RSCC, the human genes for L-lactate dehydrogenase B chain (LDHB), cyclin-dependent kinase 4 inhibit...

The AAA ATPase p97/VCP forms complexes with different adapters to fulfill distinct cellular functions. We analyzed the structural organization of the Ufd1-Npl4 adapter complex and its interaction with p97 and compared it with another adapter, p47. We found that the binary Ufd1-Npl4 complex forms a heterodimer that cooperatively interacts with p97 via a bipartite binding mechanism. Binding site ...