Mitomycin C induces both MC-mono-dG and cross-linked dG-adducts in vivo. Interstrand cross-linked (ICL) dG-MC-dG-DNA adducts can prevent strand separation. In Escherichia coli cells, UvrABC repairs ICL lesions that cause DNA bending. The mechanisms and consequences of NER of ICL dG-MC-dG lesions that do not induce DNA bending remain unclear. Using DNA fragments containing a MC-mono-dG or an ICL...

Using techniques due to Dwyer–Greenlees–Iyengar we construct weight structures in triangulated categories generated by compact objects. We apply our result to show that, for a dg category whose homology vanishes in negative degrees and is semi-simple in degree 0, each simple module over the homology lifts to a dg module which is unique up to isomorphism in the derived category. This allows us, ...

Endometrial cancer is one of the most common gynecological malignancies affecting post-menopausal women, yet the underlying mechanisms are not well understood. Dystroglycan (DG) is a large glycoprotein, consisting of α- and β-subunits that are non-covalently associated with each other. Modifications to α-DG have been linked to a variety of cancers, where the N-terminus of α-DG (α-DG-N) is post-...

【Introduction】 8-Oxo-2 ́-deoxyguanosine (8-oxo-dG) is a representative nucleoside damage that is formed by oxidation of 2 ́-deoxyguanosine (dG) with reactive oxygen species (ROS), and its presence has been linked to aging, cancer, etc [1]. Unlike dG, 8-oxo-dG forms stable base pairs with both 2 ́-deoxycytidine (dC) and 2 ́-deoxyadenosine (dA). Based on the base-pairing properties of 8-oxo-dG, DNA p...

We have solved the structures of the homoduplex d(Gm5CGCGCG)2, and the heteroduplexes d(GCGCGCG)/d(TCGCGCG) and d(GCGCGCG)/d(CCGCGCG). The structures form six base-pairs of identical Z-DNA duplexes with single nucleotides overhanging at the 5'-ends. The overhanging nucleotide from one strand remains stacked and sandwiched between the blunt-ends of two adjacent Z-DNA duplexes, while the overhang...

Proton 2D-NOE spectroscopy has been used to investigate the three-dimensional conformations of several sonicated polydeoxynucleotides in solution. The observed pattern of cross peaks indicate that poly(dAdT) . poly(dAdT1 in all salt concentrations studied (up to 6.6M CsF), and poly(dG-m5dC) . poly(dG-m5dC) in low salt (0.1M NaCU are righthanded B-structures. Poly(dG-m5dC) . poly(dG-m5dC) in Mg2...

10.1601/nm.2199 CLST is an extremely acidophilic gamma-proteobacteria that was isolated from the Gorbea salt flat, an acidic hypersaline environment in northern Chile. This kind of environment is considered a terrestrial analog of ancient Martian terrains and a source of new material for biotechnological applications. 10.1601/nm.2199 plays a key role in industrial bioleaching; it has the capaci...