Recombinant proteins are widely used as biopharmaceuticals, but their production by mammalian cell culture is expensive. Hence, improvement of bioprocess productivity is greatly needed. A temperature downshift (TDS) from 37°C to 28-34°C is an effective strategy to expand the productive life period of cells and increase their productivity (qp). Here, TDS in Chinese hamster ovary (CHO) cell cultu...

In this article, we have developed models for threading dislocation ~TD! reduction due to the introduction of an intentionally strained layer. Three different types of dislocations have been considered in this model: misfit dislocations ~MDs!, mobile TDs, and TDs whose glide motion has been blocked by a MD crossing the glide path of the TD ~immobile TDs!. The models are based on MD formation by...

A parameter called TDS (Total Dermatoscopic Score), calculated by the wellknown ABCD formula, is frequently used in melanoma diagnosis. In our previous research we found a new formula, similar to the original ABCD formula, that yielded fewer diagnostic errors. This new ABCD formula was developed using data mining techniques, in particular, the rule induction algorithm LEM2, a part of the data m...

Temperature Discovery Search (TDS) is a forward search method for computing or approximating the temperature of a combinatorial game. Temperature and mean are important concepts in combinatorial game theory, which can be used to develop efficient algorithms for playing well in a sum of subgames. A new algorithm TDS+ with five enhancements of TDS is developed, which greatly speeds up both exact ...

Coxsackievirus B3 (CVB3) generates 5'-terminally deleted genomes (TDs) during replication in murine hearts. We show here that CVB3 populations with TDs can also be generated within two to three passages of CVB3 in primary, but not immortalized, cell cultures. Deletions of less than 49 nucleotides increase in size during passage, while 5' TDs of 49 nucleotides appear to be the maximum deletion s...

We discuss the temporal efficiency of template-directed polymer synthesis, such as DNA replication and transcription, under a given template string. To weigh the synthesis speed and accuracy on the same scale, we propose a template-directed synthesis (TDS) rate, which contains an expression analogous to that for the Shannon entropy. Increasing the synthesis speed accelerates the TDS rate, but t...

We have employed an atomic force microscope with a high sampling rate to image GaN samples grown using an epitaxial layer overgrowth technique and treated with silane and ammonia to enlarge the surface pits associated with threading dislocations (TDs). This allows TDs to be identified in high pixel density images tens of microns in size providing detailed information about the spatial distribut...

Background Transient depressive state (TDS) is a transient, negative emotional state caused by certain events or situations. Because of the similarity in depressive symptoms between depression and TDS that arise within 2 weeks of their onset, it is difficult to distinguish TDS from depression. The aims of the present study were to investigate the application value of selected serum indicators i...

The commonest reproductive disorders in human males at birth (cryptorchidism, hypospadias) or young adulthood (low sperm counts, testis germ cell cancer) may comprise a ‘testicular dysgenesis syndrome’ (TDS) resulting from fetal testis dysfunction. We and others have developed an animal model for TDS, involving in utero exposure of pregnant rats to di-n-butyl phthalate (DBP), which is a common ...

BACKGROUND Tumor deposits (TDs) were reported to be poor prognoses in colorectal carcinoma, but the significance in locally advanced rectal cancer (LARC) (T3-4/N+) following neoadjuvant chemoradiotherapy (neo-CRT) and surgery is unclear. Since adjuvant chemotherapy showed no benefit for LARC following neo-CRT, it is of great value to investigate whether TDs can identify the subgroup of patients...