نتایج جستجو برای: شکستگی تک رشته ای ssb
تعداد نتایج: 262177 فیلتر نتایج به سال:
in this commentary, we argue for the implementation of a sugar-sweetened beverage (ssb) tax as a tool to help address the global obesity and diabetes epidemics. consumption of ssbs has increased exponentially over the last several decades, a trend that has been an important contributor to the obesity and diabetes epidemics. prior evidence demonstrates that a ssb tax will likely decrease ssb con...
توسعه کارست (karstification ) از جمله پدیده های زمین شناسی است که به دلیل هوازدگی و یا فرسایش شیمیایی همراه آب در مواد حل می شود، همانند سنگ آهک، دولومیت، ژیبس یا نمک در نزدیک سطح زمین . توسعهکارست غالبا در مناطقی که پوشش عظیمی از سنگ آهک، درز و شکاف وجود دارد و مناطق مرطوب به وجود می آید.محدوده تاقدیس پابده لالی بخشی از رشته کوه زاگرس در جنوب غرب ایران جهت مطالعه ارتباط کارست وشکستگی های موجود...
We have examined the ability of the Escherichia coli single-stranded DNA binding protein (SSB) tetramer to form its different binding modes on poly(dC), poly(U), and poly(A) over a range of NaCl and NaF concentrations for comparison with previous studies with poly(dT). In reverse titrations with poly(U) and poly(A) at 25 degrees C, pH 8.1, SSB forms all four binding modes previously observed wi...
Escherichia coli single-stranded DNA (ssDNA) binding protein (SSB) is the defining bacterial member of ssDNA binding proteins essential for DNA maintenance. SSB binds ssDNA with a variable footprint of ∼30-70 nucleotides, reflecting partial or full wrapping of ssDNA around a tetramer of SSB. We directly imaged single molecules of SSB-coated ssDNA using total internal reflection fluorescence (TI...
OBJECTIVE A tax on sugar-sweetened beverages (SSB) has been proposed to address population weight gain but the effect across socio-economic position (SEP) is unclear. The current study aimed to clarify the differential impact(s) of SSB taxes on beverage purchases and consumption, weight outcomes and the amount paid in SSB taxes according to SEP. DESIGN Databases (OVID and EMBASE) and grey lit...
Recently, we developed an improved comet assay protocol for evaluating single-strand break repair capacity (SSB-RC) in unstimulated cryopreserved human peripheral blood mononuclear cells (PBMCs). This methodology facilitates control of interexperimental variability [A.R. Trzeciak, J. Barnes, M.K. Evans, A modified alkaline comet assay for measuring DNA repair capacity in human populations. Radi...
Background: In prospective studies, sugar-sweetened beverages (SSB) have been associated with weight increase. However, most prospective studies examine changes in body weight over time according to amounts of SSB intake at baseline, generally without considering changes over time in SSB, energy intake and physical activity. The objective of the present study was to examine how SSB intakes infl...
The tetrameric Escherichia coli single-stranded DNA (ssDNA) binding protein (Ec-SSB) functions in DNA metabolism by binding to ssDNA and interacting directly with numerous DNA repair and replication proteins. Ec-SSB tetramers can bind ssDNA in multiple DNA binding modes that differ in the extent of ssDNA wrapping. Here, we show that the structurally similar SSB protein from the malarial parasit...
During DNA replication in Escherichia coli, single-stranded DNA-binding protein (SSB) protects single-stranded DNA from nuclease action and hairpin formation. It is known that the highly conserved C-terminus of SSB contacts the χ subunit of DNA polymerase III. However, there only exists a theoretical model in which the 11 C-terminal amino acids of SSB have been docked onto the surface of χ. In ...
The single-stranded DNA binding protein of Escherichia coli (SSB) stimulates recA protein promoted DNA strand exchange reactions by promoting and stabilizing the interaction between recA protein and single-stranded DNA (ssDNA). Utilizing the intrinsic tryptophan fluorescence of SSB, an ATP-dependent interaction has been detected between SSB and recA-ssDNA complexes. This interaction is continuo...
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