نتایج جستجو برای: سلول hek 293t

تعداد نتایج: 19597  

Background: Cutaneous leishmaniasis (CL) is a serious public health problem in many tropical countries. The infection is caused by a protozoan parasite of Leishmania genus transmitted by Phlebotominae sandflies. In the present study, we constructed a eukaryotic expression vector to produce a fusion protein containing LmSTI1 from Leishmania major (L. major) and PpSP42 from Phlebotomus papatasi (...

ژورنال: :سلول و بافت 0

هدف: هدف از این مطالعه بررسی تاثیر dj-1 بر افزایش بقای سلول‏های دوپامین ساز در برابر سمیت پارکینسونی  می‏باشد. مواد و روش‏ها:  ابتدا لنتی ویروس‏های نوترکیب ناقل توام ژن های dj-1 و گزارش‏گر jred تولید شده و برای آلوده سازی سلول‏ها استفاده شدند. بدین منظور سه پلاسمید لنتی ویروسی به‏نام‏های ناقل، بسته بندی و غشا برای ترانسفکشن سلول‏های hek-293t به‏عنوان رده سلولی مولد ویروس به‏کار برده شدند. محیط ...

Journal: :Organic letters 2011
Minhee Lee Matthew S Tremblay Steffen Jockusch Nicholas J Turro Dalibor Sames

Efficient intermolecular energy transfer from carbostyril 124-sensitized Tb(3+) to Eu(3+) in aqueous aggregates is reported. This energy transfer was also recapitulated on the cell surface of a human kidney cell line (HEK-293T) and imaged by fluorescence microscopy as an example for the applicability of this energy transfer probe for imaging in biological systems.

Journal: :Molecular and cellular endocrinology 2008
David G Mottershead Minna M Pulkki Pranuthi Muggalla Arja Pasternack Minna Tolonen Samu Myllymaa Olexandr Korchynskyi Yoshihiro Nishi Toshihiko Yanase Stan Lun Jennifer L Juengel Mika Laitinen Olli Ritvos

Growth differentiation factor-9 (GDF9) is an oocyte secreted paracrine factor essential for mammalian ovarian folliculogenesis. Like other members of the transforming growth factor-beta (TGFbeta) superfamily, GDF9 is synthesized as a prepropeptide which needs processing by furin-like proteases to result in an active mature protein. We have previously characterized a preparation of unpurified re...

2016
Ki Mo Lee Hee Kyoung Joo Yu Ran Lee Myoung Soo Park Gun Kang Sunga Choi Kwon Ho Lee Byeong Hwa Jeon

BACKGROUND Ulmus davidiana var. japonica Rehder (UD) has long been used in traditional folk medicine in Asia. This study is designed to investigate the antiadhesive activity of the ethanol extract of UD (UDE) and its underlying mechanisms in cultured endothelial cells. METHODS The dried root bark of UD was extracted with 80% (v/v) ethanol. The antiadhesive activity of the UDE was investigated...

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