Trans-3-chloroacrylic acid dehalogenase (CaaD) is a critical enzyme in the trans-1,3-dichloropropene (DCP) degradation pathway in Pseudomonas pavonaceae 170. This enzyme allows bacteria to use trans-DCP, a common component in commercially produced fumigants, as a carbon source. CaaD specifically catalyzes the fourth step of the pathway by cofactor-independent dehalogenation of a vinyl carbon-ha...

OBJECTIVE The effects of paraoxonase (PON1) activity and of genetic variation in the PON1 promoter and coding region on carotid artery disease (CAAD) were investigated. METHODS AND RESULTS We identified functional promoter polymorphisms and examined their effects in a cohort with and without CAAD. We used the full sequences in 23 white subjects to determine the linkage disequilibrium (LD) str...

Phospholipid transfer protein (PLTP) belongs to the lipid transfer/lipopolysaccharide-binding protein gene family. Expression of PLTP has been implicated in the development of atherosclerosis. We evaluated the effects of PLTP region tagging single nucleotide polymorphisms (SNPs) on the prediction of both carotid artery disease (CAAD) and PLTP activity. CAAD effects were evaluated in 442 Caucasi...

Isomer-specific 3-chloroacrylic acid dehalogenases function in the bacterial degradation of 1,3-dichloropropene, a compound used in agriculture to kill plant-parasitic nematodes. The crystal structure of the heterohexameric trans-3-chloroacrylic acid dehalogenase (CaaD) from Pseudomonas pavonaceae 170 inactivated by 3-bromopropiolate shows that Glu-52 in the alpha-subunit is positioned to funct...

This paper introduces a reflective perspective on the role of computing in architectural design over the generations of computer aided architectural designs. Paradigms of the design process and computational models of designing are discussed. The evolution of computer aided architectural design is investigated. Both deadly sins and arguable virtues of computing in architecture and their implica...

This thesis reports the author’s work within the BAS•CAAD project, and is part of the total report on the project. Property-oriented systems are a new kind of information systems that are based on concepts of properties instead of concepts of things or classes of things. By focusing on properties, the property-oriented systems become more flexible and more suited to the dynamic early stages of ...

Abstract Over the past years, computational methods based on deep learning—that is, machine learning with multilayered neural networks—have become state-of-the-art in main research areas computer-aided architectural design (CAAD). To understand current trends of CAAD learning, to situate them a broader historical context, and identify future challenges, this article presents systematic review p...

The soil of potato fields in The Netherlands harbors bacteria with the ability to metabolize 3-chloroacrylic acid, generated by the degradation of a pesticide (1,3-dichloropropene) that entered the environment in 1946. From examination of rate constants at elevated temperatures, we infer that the half-time at 25 degrees C for spontaneous hydrolytic dechlorination of trans-3-chloroacrylic acid i...

This paper reports on an integration of concept mapping for manipulating and structuring knowledge with multimedia presentation technology. It presents the current development of the HyperMedia Activated Network authoring environment. HAN is an intelligent hypertext demonstrator system. The first HAN module, called ICADT, was developed initially to teach Computer Aided Architectural Design (CAA...