a Neuroscience & Psychiatry Unit, Stopford Building, The University of Manchester, Oxford Road, Manchester M13 9PL, UK b Centre for Biostatistics, Jean McFarlane Building, The University of Manchester, Oxford Road, Manchester M13 9PL, UK c Brain Mapping Unit, Herchel Smith Building for Brain and Mind Sciences, University of Cambridge, Robinson Way, Cambridge CB2 0SZ, UK d Imaging Sciences, Stop...

Based on MAGIC observations from June and July 2007, we present upper limits to the E > 140 GeV emission from the globular cluster M13. Those limits allow us to constrain the population of millisecond pulsars within M13 and to test models for acceleration of leptons inside their magnetospheres and/or surrounding. We conclude that in M13 either millisecond pulsars are fewer than expected or they...

Commonly used in biotechnology applications, filamentous M13 phage are non-lytic viruses that infect E. coli and other bacteria, with the potential to promote horizontal gene transfer in natural populations with synthetic biology implications for engineering community systems. Using the E. coli strain TG1, we have investigated how a selective pressure involving elevated levels of toxic chromate...

Cloning of DNA fragments in phage M13 is a routine procedure in DNA sequence determination. A simple procedure for the determination of DNA fragment orientation has been described earlier (1). Here we introduce a modification of this procedure which allows the simultaneous determination of DNA fragment orientation and size. Procedure: Single-stranded M13 DNAs (1, about lpg) were mixed in 5ul of...

A new cloning strategy is described which utilizes direct selection of recombinants for shotgun sequencing in the filamentous bacteriophage M13. Direct selection is accomplished by insertional inactivation of the M13 gene X protein, a powerful inhibitor of phage-specific DNA synthesis when overproduced. An extra copy of gene X was inserted into the intergenic region of M13 and placed under the ...

DNA based molecular markers allow precise, objective and rapid cultivar identification. In this study, five ISSR and two sets of fluorescence-labeled TP-M13-SSR markers were applied for discrimination of eight closely related wheat cultivars. Five ISSR primers revealed a total of 43 distinct reproducible bands, 29 (67.44%) of the 43 bands were polymorphic. The number of polymorphic bands detect...

Representation of subcloned Caenorhabditis elegans and human DNA sequences in both M13 and pUC sequencing vectors was determined in the context of large scale genomic sequencing. In many cases, regions of subclone under-representation correlated with the occurrence of repeat sequences, and in some cases the under-representation was orientation specific. Factors which affected subclone represent...

Spectra with a high signal-to-noise ratio of 50 stars which are just below the main sequence turnoff and are members of M13 are presented. They yield indices for the strength of the CH and the ultraviolet CN band. There is no evidence for a variation in the strength of either feature from star to star in this intermediate-metallicity galactic globular cluster, and thus no evidence for primordia...

The unsymmetrical cyanine dye thiazole orange homodimer (TOTO) binds to single-stranded DNA (ssDNA, M13mp18 ssDNA) to form a fluorescent complex that is stable under the standard conditions of electrophoresis. The stability of this complex is indistinguishable from that of the corresponding complex of TOTO with double-stranded DNA (dsDNA). To examine if TOTO exhibits any binding preference for ...