The levels of cellular reactive oxygen species (ROS) and ATP as well as the mitochondrial membrane potential (MMP) in response to bovine herpesvirus 1 (BHV-1) infection of MDBK cells were measured, respectively. BHV-1 infection increased ROS production which depended on viral entry, and de novo protein expression and/or DNA replication. Vice versa, excessive ROS was required for efficient viral...

At times after conjunctival inoculation with bovine herpesvirus type 1 (BHV-1), representing the acute and latent phases of infection, rabbit trigeminal ganglia were examined for the presence of BHV-1 nucleic acids by in situ hybridization using a 3H-labelled BHV-1 DNA probe. During the acute phase of virus infection, both BHV-1 DNA and RNA were detected in ganglionic neurons and occasionally i...

The prevalence of BHV-1 and BLV antibodies was studied in 313 serum samples collected from 5 dairy cattle herds. Serum samples were collected from both apparently healthy animals and animals with respiratory symptoms. The samples were tested for the presence of antibodies against BHV-1 and BLV using virus neutralization and agar gel immunodiffusion test, respectively. The results indicated that...

Twelve Brazilian isolates and three reference strains of bovine herpesviruses (BHVs) were subjected to restriction endonuclease analysis (REA) and monoclonal antibody (MAb) analysis. Viral DNA was cleaved with BamHI, BstEII, EcoRI, HindIII and PstI. The monoclonal antibody panel allowed the differentiation between types 1 and 5 viruses, while REA with BstEII and HindIII showed the distinction b...

Bovine herpesvirus 1 (BHV-1) induces immune suppression, but the mechanisms for suppression are not well identified. We examined the induction and activity of BHV-1-specific cytolytic CD4(+) T lymphocytes (CTL) by stimulating peripheral blood mononuclear cells (PBMC) of cattle immunized with attenuated live BHV-1. Cytolytic effector cells were primarily CD4(+) T lymphocytes and lysed autologous...

OBJECTIVE To evaluate the efficacy of an inactivated bovine herpesvirus-1 (BHV-1) vaccine to protect against BHV-1 challenge-induced abortion and stillbirth. DESIGN Prospective study. ANIMALS 35 beef heifers. PROCEDURES Before breeding, heifers were vaccinated with a commercially available BHV-1 inactivated vaccine SC or IM. The estrous cycle was then synchronized, and heifers were artifi...

در این پژوهش مسئله زمان‌بندی کار کارگاهی منعطف با ماشین‌های موازی درنظرگرفتن معیار تولید پاک‌تر، منابع دوگانه انسان-ماشین، زمان دسترسی کارها و پردازش وابسته به سرعت ماشین‌ها بررسی می­‌شود. اهداف شامل حداقل­‌کردن مجموع جریمه‌­های دیرکرد زودکرد افزایش است. داده می‌شود تا تکمیل کاهش یابد. درحالی‌که آلودگی صوتی محیط تولیدی منجر توجه رویکرد پاک‌تر که نگرشی پیشگیرانه است، اینجا سعی شده است حداقل­کردن...

Identifying cellular genes that promote bovine herpesvirus-1 (BHV-1) productive infection is important, as BHV-1 is a significant bovine pathogen. Previous studies demonstrated that BHV-1 DNA is not very infectious unless cotransfected with a plasmid expressing bICP0, a viral protein that stimulates expression of all classes of viral promoters. Based on these and other studies, we hypothesize t...